Maize ubiquitin promoters

Patent metadata

Field	Value
Publication number	US-10030247-B2
Application number	US-201414587750-A
Country	US
Kind code	B2
Filing date	Dec 31, 2014
Priority date	Dec 31, 2013
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

The Zea mays c.v. B73 Ubiquitin-1 ( Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.

First claim

What is claimed is:

1. A construct comprising a promoter sequence operably linked to a heterologous nucleic acid sequence, wherein the promoter sequence comprises SEQ ID NO: 2.

2. A construct comprising the complement to the promoter sequence of SEQ ID NO: 2 operably linked to a heterologous nucleic acid sequence.

3. The construct of claim 1 , wherein the operably linked heterologous nucleic acid sequence encodes a heterologous polypeptide.

4. A gene expression cassette comprising the construct of claim 1 operably linked to a 3′-untranslated region.

5. The gene expression cassette of claim 4 , wherein the operably linked heterologous nucleic acid sequence encodes a polypeptide that confers a trait selected from the group consisting of insecticidal resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, nutritional quality, DNA binding, and selectable marker.

6. A recombinant vector comprising the gene expression cassette of claim 4 .

7. The recombinant vector of claim 6 , wherein the vector is selected from the group consisting of a plasmid vector, a cosmid vector, and a BAC vector.

8. A transgenic cell comprising the construct of claim 1 .

9. The transgenic cell of claim 8 , wherein the transgenic cell is a transgenic plant cell.

10. A transgenic plant comprising the transgenic plant cell of claim 9 .

11. A transgenic seed comprising the construct of claim 1 .

12. The transgenic plant of claim 10 , wherein the transgenic plant is a monocotyledonous transgenic plant or a dicotyledonous transgenic plant.

13. A transgenic plant tissue comprising the construct of claim 1 .

14. The transgenic plant tissue of claim 13 , wherein the transgenic plant tissue is selected from the group consisting of a root, a shoot, a stem and a pollen tissue.

15. The transgenic plant of claim 12 , wherein the transgenic plant is a monocotyledonous transgenic plant.

16. A construct comprising a recombinant polynucleotide, sequence comprising:
a first promoter sequence operably linked to a first heterologous nucleic acid sequence; and
a second promoter sequence operably linked to a second heterologous nucleic acid sequence, wherein said first promoter comprises SEQ ID NO: 1 and the second promoter comprises SEQ ID NO: 2.

17. The transgenic plant of claim 15 , wherein the monocotyledonous transgenic plant is selected from the group consisting of a maize plant, a wheat plant, and a rice plant.

18. The construct of claim 1 , wherein the operably linked heterologous nucleic acid sequence encodes a polypeptide selected from the group consisting of the Yellow Fluorescent Protein from the Phialidium species (PhiYFP) and phosphinothricin-N-acetyl-transferase (PAT).

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Gupta Manju
• Wright Terry R
• Jayne Susan M
• Smith Doug A
• Alabed Diaa

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• A01H6/4678

  Triticum sp. [wheat] · CPC title

• A01H6/4636

  Oryza sp. [rice] · CPC title

• C12N15/8274

  for herbicide resistance · CPC title