Microorganism culture vessel, microorganism test kit, method for testing dialysate, method for culturing microorganism, method for testing microorganism and method for producing microorganism culture vessel

Patent metadata

Field	Value
Publication number	US-10030218-B2
Application number	US-201314433035-A
Country	US
Kind code	B2
Filing date	Sep 26, 2013
Priority date	Oct 4, 2012
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

To provide a microorganism culture vessel that can control existence of invading foreign matters and put a large amount of sample therein, a microorganism test kit, a method for testing a dialysate, a method for culturing the microorganism, a method for testing the microorganism and a method for producing the microorganism culture vessel. The invention includes the microorganism culture vessel, including an inlet-equipped culture bag in which a sheet-like medium is put and sealed.

First claim

What is claimed is:

1. A method for producing a microorganism culture vessel, comprising:
a) placing a culture medium through an opening of an inlet-equipped culture medium bag;
b) placing the inlet-equipped culture medium bag inside a gas-permeable sterilization bag;
c) sealing an opening of the sterilization bag without sealing the inlet-equipped culture medium bag;
d) sterilizing the culture medium by placing the sterilization bag, containing the inlet-equipped culture medium bag, in an atmosphere of a sterilizing gas, such that the sterilization gas moves into the sterilization bag and sterilizes the culture medium; and
e) sealing the opening of the inlet-equipped culture medium bag by heating the opening of the inlet-equipped culture medium bag from outside the sterilization bag and while the sterilization bag, containing the inlet-equipped culture medium bag, is kept sealed, wherein
an inner surface of the inlet-equipped culture medium bag is formed of a first raw material that can be thermally welded at a temperature lower than a temperature of a second raw material forming the inner surface of the sterilization bag among the raw materials forming the sterilization bag.

2. The method for producing the microorganism culture vessel according to claim 1 further comprising:
placing the sterilization bag in a vacuum after performing step d and before performing step e; and
eliminating the sterilizing gas remaining in the sterilization bag.

3. The method for producing the microorganism culture vessel according to claim 1 , wherein removal treatment for removing the inlet-equipped culture medium bag from the sterilization bag is applied after performing step e.

4. A method for testing a dialysate, comprising:
producing a microorganism culture vessel according to claim 1 ;
removing, from the gas-permeable sterilization bag, the inlet-equipped culture medium bag;
opening the inlet of the inlet-equipped culture medium bag;
putting the dialysate from the inlet into the inlet-equipped culture medium bag;
sealing the inlet;
culturing a microorganism by placing the culture bag under conditions in which the microorganism is cultured; and
testing existence or an amount of the cultured microorganism.

5. A method for culturing a microorganism, comprising:
producing a microorganism culture vessel according to claim 1 ;
removing, from the gas-permeable sterilization bag, the inlet-equipped culture medium bag;
opening the inlet of the inlet-equipped culture medium bag;
injecting a sample liquid from the inlet into the inlet-equipped culture medium bag;
tightly sealing the inlet; and
placing the culture bag under conditions in which the microorganism is cultured.

Assignees

• Jnc Corp

Inventors

• Onji Yuichi
• Ushiyama Masashi
• Aoki Kongen

Classifications

• C12M23/38

  Caps; Covers; Plugs; Pouring means · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• G01N2333/195

  from bacteria · CPC title

• C12M23/14 Primary

  Bags · CPC title

• Y10T29/49826

  Assembling or joining · CPC title