Device for the microstructured grafting of proteins onto a substrate

Patent metadata

Field	Value
Publication number	US-10024866-B2
Application number	US-201314385384-A
Country	US
Kind code	B2
Filing date	Mar 14, 2013
Priority date	Mar 14, 2012
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

A device for the microstructured grafting of proteins onto a substrate, comprising a substrate (7), a layer comprising a polyethylene glycol and being placed on the substrate, a matrix (10) of micromirrors for propagating the light in a first pattern and for replacing the first pattern with a second pattern. The microfluidic circuit is filled so as to bring a first aqueous solution containing a first protein into contact with the layer, a first microstructured image of the first pattern being formed on the layer to photoprint the first protein on the layer, and the microfluidic circuit is adapted to replace the first aqueous solution with a second aqueous solution containing a second protein so as to bring the second aqueous solution and the layer into contact, the first pattern being replaced with the second pattern in order to photoprint the second protein on the layer.

First claim

The invention claimed is:

1. A device for the microstructured grafting of several proteins onto a substrate, comprising:
a substrate,
a layer comprising a polyethylene glycol and being placed on the substrate,
a matrix for propagating the light in a first structured pattern and for replacing the first structured pattern with a second structured pattern,
a light source for illuminating the matrix,
an optical system for forming, on the layer, a first two-dimensional microstructured image of the first structured pattern and a second two-dimensional microstructured image of the second structured pattern,
a first container for receiving a first aqueous solution comprising a first grafting component and a first protein,
a second container for receiving a second aqueous solution comprising a second grafting component and a second protein, and
a microfluidic circuit for containing the first aqueous solution, comprising an opening for bringing the first aqueous solution into contact with the layer at the opening,
wherein the microfluidic circuit is adapted to be filled with the first aqueous solution so as to bring the first aqueous solution and the layer into contact, the first two-dimensional microstructured image of the first structured pattern being formed on the layer by means of the light source in order to photoprint the first protein on the layer,
wherein the microfluidic circuit is adapted to replace the first aqueous solution with the second aqueous solution so as to bring the second aqueous solution and the layer into contact, the first structured pattern being replaced with the second structured pattern so as to form the second two-dimensional microstructured image of the second structured pattern on the layer by means of the light source in order to photoprint the second protein on the layer, and
wherein said matrix is a planar two-dimensional matrix of micromirrors, each micromirror being individually controlled and propagating the light by reflection, so that the first and second two-dimensional microstructured images have a number of pixels equal to the number of micromirrors of the matrix.

2. The device as claimed in claim 1 , wherein said optical system is a microscope objective.

3. The device as claimed in claim 1 , wherein said light source is a laser emitting at an ultraviolet wavelength.

4. The device as claimed in claim 3 , wherein said ultraviolet wavelength is 365 nm.

5. A method for the microstructured grafting of proteins onto a substrate using a device as claimed in claim 1 and comprising:
filling the first container with a first aqueous solution comprising a benzophenone and a first protein,
filling said microfluidic circuit with said first aqueous solution so as to bring the first solution and said layer into contact, at said opening, and
forming, by means of said light source, said first two-dimensional microstructured image of said first structured pattern, on the layer, in order to photoprint said first protein on the layer.

6. The method as claimed in claim 5 , wherein said first protein is fluorescent.

7. The method as claimed in claim 5 , comprising:
filling the second container with a second aqueous solution comprising the benzophenone and a second protein,
replacing said first aqueous solution with said second aqueous solution so as to bring the second solution and said layer into contact, at said opening, and
replacing said first structured pattern with said second structured pattern so as to form the second two-dimensional microstructured image of the second structured pattern on the layer by means of the light source in order to photoprint said second protein on the layer.

8. The method as claimed in claim 5 , wherein said second protein is fluorescent.

9. The device as claimed in claim 2 , wherein said light source is a laser emitting at an ultraviolet wavelength.

10. A method for the microstructured grafting of proteins onto a substrate using a device as claimed in claim 2 and comprising:
filling the first container with a first aqueous solution comprising a benzophenone and a first protein,
filling said microfluidic circuit with said first aqueous solution so as to bring the first solution and said layer into contact, at said opening, and
forming, by means of said light source, said first two-dimensional microstructured image of said first structured pattern, on the layer, in order to photoprint said first protein on the layer.

11. A method for the microstructured grafting of proteins onto a substrate using a device as claimed in claim 3 and comprising:
filling the first container with a first aqueous solution comprising a benzophenone and a first protein,
filling said microfluidic circuit with said first aqueous solution so as to bring the first solution and said layer into contact, at said opening, and
forming, by means of said light source, said first two-dimensional microstructured image of said first structured pattern, on the layer, in order to photoprint said first protein on the layer.

12. A method for the microstructured grafting of proteins onto a substrate using a device as claimed in claim 4 and comprising:
filling the first container with a first aqueous solution comprising a benzophenone and a first protein,
filling said microfluidic circuit with said first aqueous solution so as to bring the first solution and said layer into contact, at said opening, and
forming, by means of said light source, said first two-dimensional microstructured image of said first structured pattern, on the layer, in order to photoprint said first protein on the layer.

13. The method as claimed in claim 6 , comprising:
filling the second container with a second aqueous solution comprising the benzophenone and a second protein,
replacing said first aqueous solution with said second aqueous solution so as to bring the second solution and said layer into contact, at said opening, and
replacing said first structured pattern with said second structured pattern so as to form the second two-dimensional microstructured image of the second structured pattern on the layer by means of the light source in order to photoprint said second protein on the layer.

14. The method as claimed in claim 6 , wherein said second protein is fluorescent.

15. The method as claimed in claim 7 , wherein said second protein is fluorescent.

16. The device as claimed in claim 1 , wherein the first grafting component is a benzophenone.

17. The device as claimed in claim 1 , wherein the second grafting component is a benzophenone.

Assignees

• Alveole
• Centre National De La Rech Scientifique—Cnrs
• Univ De Bordeaux Segalen
• Univ De Bordeaux Segalen

Inventors

• Studer Vincent
• Azioune Ammar

Classifications

• B01J2219/00659

  Two-dimensional arrays · CPC title

• B01J2219/0074

  Biological products · CPC title

• B01J2219/00711

  Light-directed synthesis · CPC title

• B01J2219/00626

  Covalent · CPC title

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title