Identification and characterization of a peptide affinity reagent for the detection of noroviruses in clinical samples

Patent metadata

Field	Value
Publication number	US-10024856-B2
Application number	US-201414777714-A
Country	US
Kind code	B2
Filing date	Mar 21, 2014
Priority date	Mar 21, 2013
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

Embodiments of the disclosure include methods and/or compositions for the detection of viral infection, including at least Norovirus infection. In particular embodiments, there are methods and/or compositions employing particular peptides and/or phage useful for detecting Norovirus in a sample. The sample may be from an environment or from an individual. The individual may be a mammal, including a human, cow, horse, dog, cat, pig, and so forth. Certain exemplary peptides and phage that express the peptides are identified as useful for binding to Norovirus. Such peptides and phage are provided to one or more samples in order to identify whether or not Norovirus is present in the sample.

First claim

What is claimed is:

1. A composition comprising one or both of: a peptide comprising the sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, or SEQ ID NO: 23; and a phage that encodes the peptide.

2. The composition of claim 1 , wherein the peptide is no more than about 50 amino acids in length.

3. The composition of claim 1 , wherein the peptide is labeled.

4. The composition of claim 3 , wherein the label is fluorescent, radioactive, or colored.

5. A kit comprising the composition of claim 1 .

Assignees

• Baylor College Medicine

Inventors

• Palzkill Timothy Gerald
• Estes Mary K
• Atmar Robert Legare
• Rogers Jennifer Dawn
• Ajami Nadim Jose

Classifications

• G01N33/56983 Primary

  Viruses · CPC title

• G01N2469/10

  Detection of antigens from microorganism in sample from host · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• G01N2333/08

  RNA viruses · CPC title