Nucleic acid nanostructure barcode probes

Patent metadata

Field	Value
Publication number	US-10024796-B2
Application number	US-201113882223-A
Country	US
Kind code	B2
Filing date	Oct 28, 2011
Priority date	Oct 29, 2010
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

Provided herein are, inter alia, barcode probes comprised of transiently or stably fluorescently labeled nucleic acid nanostructures that are fully addressable and able to be read using standard fluorescent microscope and methods of use thereof including methods of use as detectable labels for probes.

First claim

What is claimed is:

1. A composition comprising at least two distinct nucleic acid barcode probes that each comprise a nucleic acid nanorod bound to an antibody,
wherein at least two of the nanorods comprise a distinct barcode pattern formed by the position and color of at least two fluorescently-labeled regions that are separated from each other by a fixed distance of less than 200 nm, and
wherein each fluorescently-labeled region comprises a docking strand capable of binding to a fluorescently-labeled imager strand.

2. The composition of claim 1 , wherein the docking strand is transiently bound to the fluorescently-labeled imager strand.

3. The composition of claim 1 comprising at least 10 distinct nucleic acid barcode probes.

4. The composition of claim 3 comprising at least 50 distinct nucleic acid barcode probes.

5. The composition of claim 1 , wherein each nanorod comprises at least three fluorescently-labeled regions separated from each other by a fixed distance of less than 200 nm.

6. The composition of claim 1 , wherein each fluorescently-labeled region comprises at least two docking strands, and wherein each docking strand is transiently bound to a fluorescently-labeled imager strand.

7. The composition of claim 1 , wherein the nanorod of at least one of the barcode probes comprises a first fluorescently-labeled region separated from a second fluorescently-labeled region by a fixed distance of less than 200 nm,
wherein the first fluorescently-labeled region comprises a docking strand transiently bound to a fluorescently-labeled imager strand containing a first fluorophore, and the second fluorescently-labeled region comprises a docking strand transiently bound to a fluorescently-labeled imager strand containing a second fluorophore, and
wherein the color of the first fluorophore is different from the color of the second fluorophore.

8. The composition of claim 1 , wherein the nanorod of at least one of the barcode probes comprises a first fluorescently-labeled region separated from a second fluorescently-labeled region by a fixed distance of less than 200 nm,
wherein the first fluorescently-labeled region comprises a docking strand transiently bound to a fluorescently-labeled imager strand containing a first fluorophore, and the second fluorescently-labeled region comprises at least two docking strands, each docking strand transiently bound to a fluorescently-labeled imager strand containing a second fluorophore.

9. The composition of claim 8 , wherein the color of the first fluorophore is different from the color of the second fluorophore.

10. The composition of claim 1 , wherein the nanorod of at least one of the barcode probes comprises a first fluorescently-labeled region, a second fluorescently-labeled region, and a third fluorescently-labeled region, and the distance between the first fluorescently-labeled region and second fluorescently-labeled region is shorter than the distance between the second fluorescently-labeled region and third fluorescently-labeled region.

11. The composition of claim 1 , wherein the nanorod of at least one of the barcode probes has a length of at least 200 nm.

12. The composition of claim 1 further comprising a sample.

13. The composition of claim 1 , wherein the nucleic acid barcode probes are immobilized.

Assignees

• Lin Chenxiang
• Li Chao
• Shih William M
• Yin Peng
• Jungmann Ralf
• Harvard College
• Dana Farber Cancer Inst Inc

Inventors

• Lin Chenxiang
• Li Chao
• Shih William M
• Yin Peng
• Jungmann Ralf

Classifications

• C12Q1/6825 Primary

  Nucleic acid detection involving sensors · CPC title

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N21/6486 Primary

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• C12Q2563/185

  Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals · CPC title

• C12Q2563/157

  Nanotubes or nanorods · CPC title