Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
US-9222112-B2 · Dec 29, 2015 · US
US10023884B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10023884-B2 |
| Application number | US-201414760290-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 17, 2014 |
| Priority date | Jan 18, 2013 |
| Publication date | Jul 17, 2018 |
| Grant date | Jul 17, 2018 |
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According to the present invention, a microorganism belonging to the genus Aurantiochytrium having an 18S rRNA gene consisting of the base sequence represented by any of SEQ ID NOS: 1 to 5; Aurantiochytrium sp. OH4 strain; a microorganism which is a mutant obtained from the above-mentioned microorganism as a parent strain and has a higher ability to produce DHA than the parent strain; or Aurantiochytrium sp. LTR23 strain is provided. Also, a method for producing a DHA-containing composition, DHA and DHA alkyl ester by a fermentation process using the above-mentioned microorganisms is provided.
Opening claim text (preview).
The invention claimed is: 1. A method for producing a docosahexaenoic acid-containing composition, comprising culturing Aurantiochytrium sp. OH4 strain (Accession Number: FERM BP-11524) in a medium, allowing the docosahexaenoic acid-containing composition to be produced and accumulated in a culture, and collecting the docosahexaenoic acid-containing composition from the culture. 2. A method for producing docosahexaenoic acid comprising separating and collecting docosahexaenoic acid from the docosahexaenoic acid-containing composition collected according to claim 1 . 3. A method for producing docosahexaenoic acid alkyl ester comprising separating and collecting docosahexaenoic acid alkyl ester from the docosahexaenoic acid-containing composition collected according to claim 1 . 4. A method for producing docosahexaenoic acid alkyl ester according to claim 3 , wherein the docosahexaenoic acid alkyl ester is docosahexaenoic acid methyl ester. 5. A method for producing docosahexaenoic acid alkyl ester according to claim 3 , wherein the docosahexaenoic acid alkyl ester is docosahexaenoic acid ethyl ester.
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