Production method for non-enveloped virus particles
US-2017166871-A1 · Jun 15, 2017 · US
Production method for non-enveloped virus particles
US10023846B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10023846-B2 |
| Application number | US-201515323811-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 9, 2015 |
| Priority date | Jul 10, 2014 |
| Publication date | Jul 17, 2018 |
| Grant date | Jul 17, 2018 |
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Abstract
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Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing a non-enveloped virus particle, the method comprising: (a) a step of treating a sample containing a non-enveloped virus particle with a first concentration of polyethylene glycol (PEG), (b) a step of removing a precipitate produced in step (a) and treating a supernatant with a second concentration of PEG, and (c) a step of obtaining a non-enveloped virus particle from a precipitate produced in step (b), wherein the non-enveloped virus is adeno-associated virus, and the second concentration of PEG is higher than the first concentration of PEG. 2. The method according to claim 1 , wherein the first concentration of PEG is 0.5 to 4%. 3. The method according to claim 1 , wherein the second concentration of PEG is 3 to 10%. 4. The method according to claim 1 , wherein the sample containing a non-enveloped virus particle is a crude extract obtained by bringing a non-enveloped virus-producing cell into contact with an acidic solution. 5. The method according to claim 1 , wherein the sample containing a non-enveloped virus particle is a sample treated with nuclease. 6. The method according to claim 1 , wherein the sample containing a non-enveloped virus particle is a sample treated with chloroform. 7. The method according to claim 1 , wherein the sample containing a non-enveloped virus particle is a crude extract obtained by bringing a non-enveloped virus-producing cell into contact with an acidic solution and then treated with an acid. 8. The method according to claim 1 , which comprises a step of treatment with chloroform after the step (c). 9. The method according to claim 4 , wherein the acidic solution contains a cation and citric acid. 10. The method according to claim 1 , wherein the first concentration of PEG is 0.5 to 4%, and the second concentration of PEG is 3 to 10%.
Assignees
Inventors
Classifications
- C12N7/00Primary
Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title
Methods of production or purification of viral material · CPC title
Viral vectors · CPC title
Recovery or purification · CPC title
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- What does patent US10023846B2 cover?
- Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingr…
- Who is the assignee on this patent?
- Takara Bio Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N7/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 17 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).