Device for processing a liquid medium comprising cells

Patent metadata

Field	Value
Publication number	US-10022732-B2
Application number	US-201415035711-A
Country	US
Kind code	B2
Filing date	Dec 17, 2014
Priority date	Jan 27, 2014
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

The invention relates to a device for processing an initial liquid medium (4) comprising cells in an initial concentration in order to obtain a product liquid medium (4′) comprising cells in a product concentration, the device (2) comprising a separator (6) adapted to separate the initial liquid medium (4) into various constituents, a first supply system (8) for supplying the initial liquid medium (4) to the separator (6), a first outlet system (12) for extracting the product liquid medium (4′) from the separator (6), a sensor (22, 22′) adapted to measure a physical parameter related to the concentration of the cells in the initial or product liquid medium (4, 4′), and a control unit (24) coupled to the sensor (22, 22′) and adapted to control at least one process of the device (2) as a function of the physical parameter measured by the sensor (22, 22′). The device is characterized by a second supply system (10) for supplying a solution (18) to the separator (6) with a given flow rate. The flow rate of the solution (18) in the second supply system (10) during its supply to the separator (6) is determined based on the at least one process parameter.

First claim

The invention claimed is:

1. A device for processing an initial liquid medium comprising cells in an initial concentration in order to obtain a product liquid medium comprising cells in a product concentration, the device comprising:
a separator adapted to separate the initial liquid medium into various constituents,
a first supply system for supplying the initial liquid medium to the separator,
a first outlet system for extracting the product liquid medium from the separator,
a sensor adapted to measure a physical parameter related to the concentration of the cells in the initial liquid medium,
central processing unit coupled to the sensor and adapted to control at least one process parameter of the device as a function of the physical parameter measured by the sensor, and
a second supply system for supplying a solution to the separator with a given flow rate wherein the flow rate of the solution in the second supply system during its supply to the separator is determined based on the at least one process parameter
wherein the central processing unit is configured to reduce the flow rate of the solution in the second supply system when the concentration of the cells measured in the initial liquid medium increases.

2. The device according to claim 1 , wherein the at least one process parameter determines the flow rate of the initial liquid medium in the first supply system during its supply to the separator and/or the flow rate of the product liquid medium in the first outlet system during its extraction from the separator.

3. The device according to claim 2 , wherein the central processing unit is configured to control the flow rate of the initial liquid medium such that it is indirectly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system and/or the flow rate of the product liquid medium such that it is directly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

4. The device according to claim 1 , wherein the separator is a centrifuge.

5. The device according to claim 4 , wherein the central processing unit is configured to control a rotational speed of the centrifuge based on the at least one process parameter such that the rotational speed is indirectly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

6. The device according to claim 1 , wherein the first supply system comprises a supply line through which the initial liquid medium flows into the separator and the sensor is adapted to measure a physical parameter related to the initial concentration of the cells in the initial liquid medium that is present in the supply line of the first supply system.

7. The device according to claim 1 , wherein the first outlet system comprises an output line through which the product liquid medium flows out of the separator and the sensor is adapted to measure a physical parameter related to the product concentration of the cells in the product liquid medium that is present in the output line of the first outlet system.

8. The device according to claim 1 , wherein the central processing unit is configured to control the at least one process parameter of the device such that the product concentration of the cells in the product liquid medium is within a predefined concentration range.

9. The device according to claim 1 , wherein the central processing unit is configured to control the at least one process parameter of the device such as to maximize the product concentration of the cells in the product liquid medium.

10. The device according to claim 1 , wherein the device is configured to operate in vitro.

11. The device according to claim 1 , further comprising a first sensor adapted to measure a physical parameter related to the concentration of the cells in the initial liquid medium and a second sensor adapted to measure a physical parameter related to the concentration of the cells in the product liquid medium.

Assignees

• Fresenius Kabi Deutschland Gmbh

Inventors

• Meisberger Artur
• Fahrendorff Melanie
• Brinkmann Michael
• Biehl Martin

Classifications

• B04B9/10 Primary

  Control of the drive; Speed regulating {(for solid bowl screw centrifuges B04B1/2016)} · CPC title

• A61M1/3616

  Batch-type treatment · CPC title

• B04B11/02 Primary

  Continuous feeding or discharging; Control arrangements therefor · CPC title

• C07D413/04

  directly linked by a ring-member-to-ring-member bond · CPC title

• C07D403/04

  directly linked by a ring-member-to-ring-member bond · CPC title