Method of manufacturing micro chamber plate with built-in sample and analytic micro chamber plate, analytic micro chamber plate and apparatus set for manufacturing analytic micro chamber plate with built-in sample

Patent metadata

Field	Value
Publication number	US-10022717-B2
Application number	US-201514875246-A
Country	US
Kind code	B2
Filing date	Oct 5, 2015
Priority date	Jul 23, 2010
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

A method of manufacturing a micro-chamber plate with a built-in sample, including: settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening; disposing a cover for micro-chamber plate receiving part to cover the upper opening, the cover for micro-chamber plate receiving part having a provisional storing part and an auxiliary covering part connected with the provisional storing part and formed with a through-hole for auxiliary covering part; and manufacturing a micro-chamber plate with a built-in sample by putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which can apply vacuum, applying centrifugal force and injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate through a vessel communication part which is formed at the provisional storing part to be communicated with the micro-chamber plate receiving part.

First claim

What is claimed is:

1. A method of manufacturing a micro-chamber plate with a built-in sample, comprising:
settling a micro-chamber plate for sample injection at a micro-chamber plate receiving part formed with an upper opening;
disposing a cover on the micro-chamber plate receiving part so as to cover the upper opening of the micro-chamber plate receiving part, the cover comprising:
a provisional storing part formed with a cutting line; and
an auxiliary covering part connected with the provisional storing part and formed with a through-hole;

putting the micro-chamber plate receiving part, on which the cover is disposed, into a centrifugal separator which is capable of applying centrifugal force and vacuum into the centrifugal separator; and
injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate for sample injection through the cutting line which is formed at the provisional storing part so as to be communicated with the micro-chamber plate receiving part.

2. The method of claim 1 , wherein the injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate comprises:
applying the vacuum into the centrifugal separator and generating the centrifugal force at a first level while the vacuum is applied into the centrifugal separator; and
releasing the vacuum in the centrifugal separator while generating the centrifugal force at a second level larger than the first level in the centrifugal separator such that the sample solution is injected into the micro-chamber plate through the cutting line.

3. The method of claim 2 , wherein the centrifugal force at the first level is configured to suppress bumping of the sample solution, while the vacuum is applied into the centrifugal separator and the cutting line is closed.

4. The method of claim 3 , wherein the cutting line is formed at the provisional storing part so as to be opened by external force, and
the centrifugal force at the second level is configured to open the cutting line.

5. A method of manufacturing an analytic micro-chamber plate using the micro-chamber plate with the built-in sample manufactured by the method of claim 1 , comprising:
taking out the micro-chamber plate with the built-in sample from the centrifugal separator and then sealing a separation membrane of the micro-chamber plate with the built-in sample.

6. A method of manufacturing a micro-chamber plate with a built-in sample, comprising:
forming a sample solution storing space between a cover for a micro-chamber plate receiving part and an upper surface of a micro-chamber plate for sample injection by closely contacting a lower end of the cover to the upper surface of the micro-chamber plate for sample injection, the cover comprising:
a provisional storing part formed with a cutting line; and
an auxiliary covering part connected with the provisional storing part and formed with a through-hole;

putting the micro-chamber plate for sample injection and the cover, which are closely contacted with each other so as to form the sample solution space therebetween, into a centrifugal separator which is capable of applying centrifugal force and vacuum into the centrifugal separator; and
injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate for sample injection through the cutting line which is formed at the provisional storing part so as to be communicated with the sample solution storing space.

7. The method of claim 6 , wherein the injecting a sample solution provisionally stored in the provisional storing part into the micro-chamber plate comprises:
applying the vacuum into the centrifugal separator and generating the centrifugal force at a first level while the vacuum is applied into the centrifugal separator; and
releasing the vacuum in the centrifugal separator while generating the centrifugal force at a second level larger than the first level in the centrifugal separator such that the sample solution is injected into the micro-chamber plate through the cutting line.

8. The method of claim 7 , wherein the centrifugal force at the first level is configured to suppress bumping of the sample solution, while the vacuum is applied into the centrifugal separator and the cutting line is closed.

9. The method of claim 8 , wherein the cutting line is formed at the provisional storing part so as to be opened by external force, and
the centrifugal force at the second level is configured to open the cutting line.

10. A method of manufacturing an analytic micro-chamber plate using the micro-chamber plate with the built-in sample manufactured by the method of claim 6 , comprising:
taking out the micro-chamber plate with the built-in sample from the centrifugal separator and sealing a separation membrane of the micro-chamber plate with the built-in sample.

Assignees

• Bioneer Corp

Inventors

• Park Han Oh
• Song Gu Young
• Bae Jung A

Classifications

• B01L3/50851

  specially adapted for heating or cooling samples · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B01L3/50255 Primary

  Multi-well filtration · CPC title

• B01L2300/044

  pierceable, e.g. films, membranes · CPC title

• B01L2300/0681

  Filter · CPC title