Elastase treatment of tissue matrices

Patent metadata

Field	Value
Publication number	US-10022473-B2
Application number	US-201614989301-A
Country	US
Kind code	B2
Filing date	Jan 6, 2016
Priority date	Jun 6, 2008
Publication date	Jul 17, 2018
Grant date	Jul 17, 2018

Abstract

A method of producing a modified acellular tissue matrix (mATM) from an acellular tissue matrix (ATM), where the mATM has a reduced stretchiness relative to the ATM, without substantially compromising its associated structural or functional integrity. The method includes providing an acellular tissue matrix (ATM) and exposing the ATM to elastase for a period of time.

First claim

The invention claimed is:

1. A method comprising:
selecting an acellular tissue matrix (ATM);
exposing the ATM to a solution containing elastase for a period of time, the exposure resulting in a modified ATM (mATM); and
controlling the exposure time and concentration of elastase in the solution to obtain a percent extension of the mATM under an amount of tensile force,
wherein the percent extension of the mATM in a toe region of the stress-strain curve resulting from an amount of tensile force is less than the percent extension of the ATM resulting from the same amount of tensile force.

2. The method of claim 1 , wherein the tissue matrix is a dermal tissue matrix.

3. The method of claim 2 , wherein the tissue matrix is a porcine tissue matrix.

4. The method of claim 2 , wherein the tissue matrix is a human tissue matrix.

5. The method of claim 2 , wherein the tissue matrix is a bovine tissue matrix.

6. The method of claim 1 , wherein the concentration of elastase ranges from about 0.1 units/milliliter to 0.5 units/milliliter.

7. The method of claim 1 , wherein the concentration of elastase ranges from about 0.2 units/milliliter to 0.25 units/milliliter.

8. The method of claim 1 , wherein the exposure time ranges from about 12 hours to 24 hours.

9. The method of claim 1 , wherein the exposure time ranges from 18 hours to 24 hours.

10. A method comprising:
providing the modified acellular tissue matrix (mATM) of claim 1 ;
identifying a vertebrate subject as having an organ or tissue in need of treatment; and
placing the mATM in or on the tissue or organ.

11. A method of treating a group of acellular tissue matrices, the method comprising:
selecting a group of acellular tissue matrices (ATMs) having variability among the ATMs in a percent extension in a toe region of a stress-strain curve; and
exposing one or more ATMs of the group to a solution containing elastase for a period of time to produce one or more modified ATMs (mATMs), wherein after exposing one or more of the ATMs to elastase, the variability among the ATMs in the percent extension in the toe region of the stress-strain curve is reduced.

12. The method of claim 11 , wherein the percent extension of a plurality of the one or more of the mATMs ranges from 14% to 24%.

13. The method of claim 11 , wherein the concentration of elastase ranges from about 0.1 units/milliliter to 0.5 units/milliliter.

14. The method of claim 11 , wherein the concentration of elastase ranges from about 0.2 units/milliliter to 0.25 units/milliliter.

15. The method of claim 11 , wherein said period of time ranges between 12 to 24 hours.

16. The method of claim 11 , wherein said period of time ranges between 18 hours to 24 hours.

17. The method of claim 11 , wherein the ATMs are made from porcine tissue.

18. The method of claim 11 , wherein the ATMs are made from human tissue.

19. The method of claim 11 , wherein the ATMs are made from bovine tissue.

20. The method of claim 11 , wherein the ATMs are dermal ATMs.

Assignees

• Lifecell Corp

Inventors

• Sun Wendell

Classifications

• F03C2/08

  of intermeshing-engagement type, i.e. with engagement of co- operating members similar to that of toothed gearing · CPC title

• A61L27/362

  Skin, e.g. dermal papillae · CPC title

• A61L27/3687 Primary

  characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents · CPC title

• A61L27/3604

  characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel · CPC title

• F01C21/06

  Heating; Cooling (of machines or engines in general F01P); Heat insulation (heat insulation in general F16L) · CPC title