System and method for retrieval treatment of proteins in formalin-fixed paraffin-embedded tissue section

Patent metadata

Field	Value
Publication number	US-10018539-B2
Application number	US-201514978115-A
Country	US
Kind code	B2
Filing date	Dec 22, 2015
Priority date	May 25, 2011
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

A protein retrieval treatment system used for activating proteins contained in a deparaffinized tissue section obtained by removing paraffin from a formalin-fixed paraffin embedded tissue section. The protein retrieval treatment system includes: a dispensing unit for dispensing a retrieval treatment solution over a dispensing area including a measurement area on the deparaffinized tissue section; and a moist-heat treatment unit for heating, in a saturated water vapor, the deparaffinized tissue section with the dispensing area covered with the retrieval treatment solution.

First claim

The invention claimed is:

1. A protein retrieval treatment method comprising:
putting a methylene crosslink dissociation treatment solution on a dispensing area including a measurement area for a mass spectrometry on a deparaffinized tissue section prepared by removing paraffin from a formalin-fixed paraffin-embedded tissue section obtained by a formalin-fixing treatment followed by a paraffin-embedding treatment, wherein the methylene crosslink dissociation treatment solution is put on the dispensing area such that the deparaffinized tissue section is not immersed in the methylene crosslink dissociation treatment solution; and
heating the deparaffinized tissue section with the methylene crosslink dissociation treatment solution put on the dispensing area by using a saturated water vapor and thereby dissociating a crosslink resulting from formalin fixation in protein molecules contained in the measurement area.

2. The protein retrieval treatment method according to claim 1 , wherein the methylene crosslink dissociation treatment solution is dispensed at predetermined space intervals so as to form a plurality of droplets separated from each other within the dispensing area.

3. The protein retrieval treatment method according to claim 2 , wherein a volume of each of the droplets and the space intervals of the droplets are set so that the dispensing area is covered with the plurality of droplets.

4. The protein retrieval treatment method according to claim 1 , wherein the methylene crosslink dissociation treatment solution is dispensed a plurality of times onto a same spot within the dispensing area.

5. The protein retrieval treatment method according to claim 1 , wherein a pH of the methylene crosslink dissociation treatment solution is equal to or higher than 9.

Assignees

• Shimadzu Corp
• Shizuoka Prefecture

Inventors

• Aoki Yutaka
• Umino Yukari
• Sato Taka-Aki
• Hayashi Isamu
• Hatakeyama Keiichi
• Ogura Shun-Ichiro

Classifications

• G01N33/6848

  Methods of protein analysis involving mass spectrometry · CPC title

• G01N33/68

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title