Rhizomania-resistant gene

Patent metadata

Field	Value
Publication number	US-10017781-B2
Application number	US-201414899416-A
Country	US
Kind code	B2
Filing date	Jun 6, 2014
Priority date	Jun 17, 2013
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the Beta genus, in which the polypeptide is expressed, and also a preferred nucleic acid molecule encoding the RZ-3 gene of Beta maritima , derivatives and homologues thereof. Further aspects of the invention include vectors, transgenic plant cells, transgenic plants, methods for production thereof, and methods for identifying a resistance-conveying nucleic acid molecule.

First claim

The invention claimed is:

1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence SEQ ID NO: 2 or SEQ ID NO: 3,
b) a nucleotide sequence comprising the sequence of SEQ ID NO: 1,
c) a nucleotide sequence that encodes a polypeptide which comprises an amino acid sequence at least 85% identical to SEQ ID NO: 2 or SEQ ID NO: 3, and
d) a nucleotide sequence that encodes a polypeptide comprising at least one nucleotide-binding domain (NBS) corresponding to amino acid positions 168-227 of SEQ ID NO: 2 or corresponding to amino acid positions 182-241 of SEQ ID NO: 3, at least one leucine-rich domain (LRR) corresponding to amino acid positions 591-613 of SEQ ID NO: 2 or corresponding to amino acid positions 605-627 of SEQ ID NO: 3, and at least one internal repetitive domain (IR) corresponding to amino acid positions 1013-1072 of SEQ ID NO: 2 or corresponding to amino acid positions 1027-1086 of SEQ ID NO: 3;
wherein the nucleic acid molecule is operably linked to a heterologous promoter.

2. A vector that comprises the isolated nucleic acid molecule according to claim 1 .

3. A plant cell that comprises a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence SEQ ID NO: 2 or SEQ ID NO: 3,
b) a nucleotide sequence comprising the sequence of SEQ ID NO: 1,
c) a nucleotide sequence that encodes a polypeptide which comprises an amino acid sequence at least 85% identical to SEQ ID NO: 2 or SEQ ID NO: 3 or to a polypeptide encoded by SEQ ID NO: 1, and
d) a nucleotide sequence that encodes a polypeptide comprising at least one nucleotide-binding domain (NBS) corresponding to amino acid positions 168-227 of SEQ ID NO: 2 or corresponding to amino acid positions 182-241 of SEQ ID NO: 3, at least one leucine-rich domain (LRR) corresponding to amino acid positions 591-613 of SEQ ID NO: 2 or corresponding to amino acid positions 605-627 of SEQ ID NO: 3, and at least one internal repetitive domain (IR) corresponding to amino acid positions 1013-1072 of SEQ ID NO: 2 or corresponding to amino acid positions 1027-1086 of SEQ ID NO: 3,
wherein said nucleotide sequence is heterologous to said cell.

4. A cell that comprises the vector according to claim 2 .

5. The cell according to claim 4 , wherein the cell is a plant cell.

6. A plant or a part thereof, comprising the plant cell according to claim 3 .

7. A plant or a part thereof, comprising the plant cell according to claim 5 .

8. A seed of a plant, wherein the seed comprises the nucleic acid molecule according to claim 1 as a transgene or as a cisgene.

9. The cell according to claim 3 , wherein the cell comprises the nucleotide sequence as an introgressed region.

10. A method for producing a transgenic plant cell, wherein the method comprises a step of introducing the nucleic acid molecule according to claim 1 into the plant cell.

11. A method for producing a transgenic plant, wherein the method comprises the following steps
a) introducing the nucleic acid molecule according to claim 1 into a plant cell, and
b) regenerating the transgenic plant from the transgenic plant cell from step a).

12. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule does not comprise a regulatory sequence comprising nucleotides 1-1403 of SEQ ID NO: 1.

13. A cell according to claim 4 wherein the cell is a host cell.

14. A seed of a plant, wherein the seed comprises the plant cell according to claim 9 .

15. A plant or part thereof, wherein the plant or part thereof comprises the plant cell according to claim 9 .

16. A method for identifying and introducing a nucleic acid molecule which encodes a protein that is able to convey a resistance to Beet Necrotic Yellow Vein Virus (BNYVV) into a plant of the Beta genus in which the protein is expressed, wherein the method comprises the following steps:
a) providing genomic DNA of a plant of the Beta genus,
b) detecting in the genomic DNA the absence of an insertion in the nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or 3 by using a molecular marker,
c) isolating the nucleotide sequence without the insertion, and
d) introducing the isolated [a]nucleotide sequence without the insertion into a plant or plant part.

17. A method for identifying a nucleic acid molecule which encodes a protein that is able to convey a resistance to Beet Necrotic Yellow Vein Virus (BNYVV) in a plant of the Beta genus in which the protein is expressed, wherein the method comprises the following steps:
a) providing genomic DNA of a plant of the Beta genus, and
b) detecting at least one polymorphism in the nucleotide sequence encoding a polypeptide comprising the amino acid sequence SEQ ID NO: 2 or SEQ ID NO: 3 by
detecting the presence or absence of a nucleotide selected from C at position 94 of SEQ ID NO: 1, T at position 95 of SEQ ID NO: 1, T at position 96 of SEQ ID NO: 1, C at position 97 of SEQ ID NO: 1, C at position 98 of SEQ ID NO: 1, T at position 99 of SEQ ID NO: 1, C at position 100 of SEQ ID NO: 1, T at position 101 of SEQ ID NO: 1, G at position 102 of SEQ ID NO: 1, T at position 103 of SEQ ID NO: 1, T at position 104 of SEQ ID NO: 1, C at position 105 of SEQ ID NO: 1, T at position 106 of SEQ ID NO: 1, G at position 107 of SEQ ID NO: 1, T at position 108 of SEQ ID NO: 1, T at position 109 of SEQ ID NO: 1, T at position 110 of SEQ ID NO: 1, T at position 111 of SEQ ID NO: 1, A at position 112 of SEQ ID NO: 1, A at position 113 of SEQ ID NO: 1, A at position 114 of SEQ ID NO: 1, A at position 118 of SEQ ID NO: 1, A at position 119 of SEQ ID NO: 1, C at position 120 of SEQ ID NO: 1, G at position 121 of SEQ ID NO: 1, T at position 123 of SEQ ID NO: 1, C at position 130 of SEQ ID NO: 1, T at position 131 of SEQ ID NO: 1, A at position 133 of SEQ ID NO: 1, G at position 135 of SEQ ID NO: 1, C at position 136 of SEQ ID NO: 1, A at position 137 of SEQ ID NO: 1, A at position 138 of SEQ ID NO: 1, C at position 139 of SEQ ID NO: 1, C at position 140 of SEQ ID NO: 1, C at position 141 of SEQ ID NO: 1, A at position 142 of SEQ ID NO: 1, A at position 143 of SEQ ID NO: 1, C at position 144 of SEQ ID NO: 1, C at position 145 of SEQ ID NO: 1, C at position 146 of SEQ ID NO: 1, A at position 147 of SEQ ID NO: 1, C at position 148 of SEQ ID NO: 1, T at position 149 of SEQ ID NO: 1, T at position 150 of SEQ ID NO: 1, T at position 151 of SEQ ID NO: 1, T at position 152 of SEQ ID NO: 1, T at position 153 of SEQ ID NO: 1, T at position 154 of SEQ ID NO: 1, A at position 155 of SEQ ID NO: 1, A at position 156 of SEQ ID NO: 1, T at position 157 of SEQ ID NO: 1, A at position 158 of SEQ ID NO: 1, A at position 159 of SEQ ID NO: 1, T at position 160 of SEQ ID NO: 1, A at position 161 of SEQ ID NO: 1, A at position 162 of SEQ ID NO: 1, A at position 163 of SEQ ID NO: 1, T at position 164 of SEQ ID NO: 1, A at position 165 of SEQ ID NO: 1, T at position 166 of SEQ ID NO: 1, T at position 167 of SEQ ID NO: 1, T at position 168 of SEQ ID NO: 1, T at position 169 of SEQ ID NO: 1, T at position 170 of SEQ ID NO: 1, A at position 171 of SEQ ID NO: 1, G at position 172 of SEQ ID NO: 1, T at position 173 of SEQ ID NO: 1, T at position 174 of SEQ ID NO: 1, G at position 175 of SEQ ID NO: 1, T at position 176 of SEQ ID NO: 1, G at position 177 of SEQ ID NO: 1, T at position 178 of SEQ ID NO: 1, G at position 179 of SEQ ID NO: 1, C at position 180 of SEQ ID NO: 1, A a

Assignees

• Kws Saat Se & Co Kgaa

Inventors

• Torjek Otto
• Borchardt Dietrich
• Mechelke Wolfgang
• Lein Jens Christoph

Classifications

• C07K14/415

  from plants · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N15/8283 Primary

  for virus resistance · CPC title

• C12Q2600/13

  Plant traits · CPC title