Interleukin-10 peptides and antibodies thereof for inhibiting adverse effects of protozoan infection

Patent metadata

Field	Value
Publication number	US-10017569-B2
Application number	US-201615334542-A
Country	US
Kind code	B2
Filing date	Oct 26, 2016
Priority date	Jul 13, 2012
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

The present disclosure is directed to interleukin-10 (IL-10) peptides and isolated antibodies that specifically bind to the IL-10 peptides. The IL-10 peptides and the isolated antibodies may be administered alone or as an animal feed additive to treat gastrointestinal protozoan infection in animals.

First claim

What is claimed is:

1. An animal feed additive comprising an isolated antibody that specifically binds to an interleukin-10 peptide, the interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

2. The animal feed additive of claim 1 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

3. The animal feed additive of claim 1 , wherein the isolated antibody is an avian egg antibody.

4. The animal feed additive of claim 3 , wherein the avian egg antibody is concentrated in the egg yolk.

5. The animal feed additive of claim 4 , wherein the egg yolk is dried.

6. The animal feed additive of claim 1 , wherein the antibody is a monoclonal antibody, a synthetic antibody, or a genetically engineered antibody.

7. An isolated antibody that specifically binds to an interleukin-10 peptide, the interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

8. The isolated antibody of claim 7 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

9. A method of generating an antibody, the method comprising administering an interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20 to an animal.

10. The method of claim 9 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

11. The method of claim 10 , wherein the generated antibody is concentrated in an egg of an avian animal.

12. The method of claim 11 , wherein the generated antibody is concentrated in an egg yolk of the egg of the avian animal.

13. The method of claim 12 , further comprising isolating the generated antibody from the egg yolk.

14. The method of claim 13 , further comprising drying the egg yolk; and introducing the dried egg yolk into an animal feed.

15. The method of claim 14 , wherein the interleukin-10 peptide is conjugated to a carrier prior to administration.

16. The method of claim 11 , further comprising drying the egg, and introducing the dried egg into an animal feed.

17. A method for treating a gastrointestinal protozoan infection in an animal, the method comprising administering an interleukin-10 peptide or an isolated antibody that specifically binds to an interleukin-10 peptide, the interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

18. The method of claim 17 , wherein the interleukin-10 peptide or isolated antibody that specifically binds to the interleukin-10 peptide is administered to the animal by injection or oral delivery.

19. The method of claim 17 , wherein the animal is selected from the group consisting of a cow, a pig, a sheep, a fish, a quail, a duck, a turkey, a pheasant and a chicken.

20. The method of claim 17 , wherein the animal is an avian animal selected from the group consisting of a chicken, turkey, duck, pheasant and quail.

21. The method of claim 17 , wherein the protozoa is an Apicomplexa.

22. The method of claim 21 , wherein the protozoa is selected from the group consisting of Cryptosporidium, Eimeria acervulina, Eimeria tenella, Eimeria maxima and Eimeria brunetti.

23. The method of claim 17 , wherein treating the gastrointestinal protozoan infection comprises maintaining growth of the animal.

24. A method for treating a gastrointestinal protozoan infection in an animal, the method comprising administering the isolated antibody that specifically binds to an interleukin-10 peptide, the interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

25. The method of claim 24 , wherein the isolated antibody that specifically binds to the interleukin-10 peptide is administered to the animal by injection or oral delivery.

26. The method of claim 24 , wherein the animal is selected from the group consisting of a cow, a pig, a sheep, a fish, a quail, a duck, a turkey, a pheasant and a chicken.

27. The method of claim 24 , wherein the animal is an avian animal selected from the group consisting of a chicken, turkey, duck, pheasant and quail.

28. The method of claim 24 , wherein the protozoa is an Apicomplexa.

29. The method of claim 28 , wherein the protozoa is selected from the group consisting of Cryptosporidium, Eimeria acervulina, Eimeria tenella, Eimeria maxima and Eimeria brunetti.

30. The method of claim 24 , wherein treating the gastrointestinal protozoan infection comprises maintaining growth of the animal.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Sand Jordan Marshall
• Cook Mark Eric

Classifications

• A61K38/2066

  IL-10 · CPC title

• C07K16/244 Primary

  Interleukins [IL] · CPC title

• A61K2039/505

  comprising antibodies · CPC title

• A23K50/75

  for poultry · CPC title

• C07K14/5428

  IL-10 · CPC title