Peptide selectively binding to graphitic materials and volatile organic compounds

Patent metadata

Field	Value
Publication number	US-10017537-B2
Application number	US-201514793032-A
Country	US
Kind code	B2
Filing date	Jul 7, 2015
Priority date	Apr 15, 2013
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

Provided is a peptide including X 2 SX 1 AAX 2 X 3 P (SEQ ID NO. 1), X 2 X 2 PX 3 X 2 AX 3 P (SEQ ID NO. 2), SX 1 AAX 2 X 3 P (SEQ ID NO. 3), or X 2 PX 3 X 2 AX 3 P (SEQ ID NO. 4), which bind to graphitic materials or volatile organic compounds.

First claim

What is claimed is:

1. A peptide or peptide set comprising
one or more amino acid sequences selected from the group consisting of SEQ ID NOs: 5 to 8, wherein the peptide or peptide set is no more than 60 amino acids in length.

2. The peptide or peptide set of claim 1 , wherein the peptide or peptide set specifically binds to a graphitic material, and wherein the graphitic material is selected from the group consisting of graphite, graphene, highly ordered pyrolytic graphite (HOPG), carbon nanotube, and fullerene.

3. The peptide or peptide set of claim 1 , wherein the peptide or peptide set is displayed on a coat protein of a phage or a fragment thereof, and wherein the C-terminus of the peptide or peptide set is linked to the N-terminus of a coat protein of the phage, or the peptide or peptide set is inserted between consecutive amino acid sequences of the coat protein of the phage or replaces the consecutive amino acid sequences of the coat protein.

4. The peptide or peptide set of claim 3 , wherein the peptide or peptide set comprising the amino acid sequences of SEQ ID NOs: 5 or 7 specifically binds to benzene, and the peptide or peptide set comprising the amino acid sequences of SEQ ID NOs: 6 or 8 specifically binds to toluene, xylene, hexane or a combination thereof.

5. The peptide or peptide set of claim 4 for use in detecting or eliminating a volatile organic compound selected from the group consisting of benzene, toluene, xylene, and hexane,
wherein the peptide or peptide set specifically binding to benzene comprises the amino acid sequences of SEQ ID NOs: 5 or 7, and the peptide or peptide set specifically binding to toluene, xylene, or hexane comprises the amino acid sequences of SEQ ID NOs: 6 or 8.

6. The peptide or peptide set of claim 1 , wherein the peptide or peptide set specifically binds to highly ordered pyrolytic graphite (HOPG).

7. The peptide or peptide set of claim 1 , wherein the peptide or peptide set comprising SEQ ID NOs: 5 or 7 specifically binds to graphene or carbon nanotube.

8. A peptide or peptide set consisting of one or more amino acid sequences selected from the group consisting of SEQ ID NOs: 5 to 8.

9. A phage, comprising the peptide or peptide set of claim 1 displayed on a coat protein of the phage or a fragment thereof.

10. The phage of claim 9 , wherein the phage is M13 phage, F1 phage, Fd phage, If1 phage, Ike phage, Zj/Z phage, Ff phage, Xf phage, Pf1 phage, or Pf3 phage.

11. The phage of claim 9 , wherein the C-terminus of the peptide or peptide set is linked to the N-terminus of a coat protein of the phage, or the peptide or peptide set is inserted between consecutive amino acid sequences of the coat protein of the phage or replaces the consecutive amino acid sequences of the coat protein.

12. The phage of claim 11 , wherein the coat protein is selected from the group consisting of p3, p6, p8 and p9 of M13 phage.

Assignees

• Korea Inst Sci & Tech

Inventors

• Yi Hyunjung
• Lee Ki Young
• Jang Chaun
• Chang Joonyeon
• Kim Sang Kyung
• Ju Soomi

Classifications

• C07K7/06 Primary

  having 5 to 11 amino acids · CPC title