Immunogenic composition in emulsion form

Patent metadata

Field	Value
Publication number	US-10016510-B2
Application number	US-201414770109-A
Country	US
Kind code	B2
Filing date	Feb 26, 2014
Priority date	Feb 26, 2013
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

The present invention relates to an immunogenic composition comprising a continuous aqueous phase and a dispersed phase as droplets and comprising:
an amphiphilic lipid, a solubilizing lipid comprising at least one fatty acid glyceride, a co-surfactant comprising at least one chain consisting of alkylene oxide units, a surfactant bearing an antigen of the following formula (I):

(L 1 -X 1 —H 1 —Y 1 ) v -G-Z 1 —Ag  (I),

to its preparation method and its uses, notably for producing antibodies, as a drug or in an immunization method.

First claim

The invention claimed is:

1. An immunogenic composition comprising a continuous aqueous phase and a dispersed phase as droplets and comprising:
an amphiphilic lipid,
a solubilizing lipid comprising at least one fatty acid glyceride,
a co-surfactant comprising at least one chain consisting of alkylene oxide units,
a surfactant bearing an antigen of the following formula (I′):

wherein:
R 2 represents a linear hydrocarbon chain comprising from 7 to 23 carbon atoms,
A 2 represents O or NH
n represents an integer from 3 to 500, and
Ag represents an antigen, and

wherein the molar ratio of the surfactant bearing an antigen of formula (I′) over the sum of the co-surfactant and of the surfactant bearing an antigen of formula (I′) is from 0.01% to 5%.

2. The immunogenic composition according to claim 1 , wherein:
the amphiphilic lipid is a phospholipid, and/or
the solubilizing lipid consists of a mixture of saturated fatty acid glycerides including at least 10% by weight of C 12 fatty acids, at least 5% by weight of C 14 fatty acids, at least 5% by weight of C 16 fatty acids and at least 5% by weight of C 18 fatty acids, and/or
the co-surfactant is selected from polyethyleneglycol/phosphatidyl-ethanolamine conjugate compounds, fatty acid and polyethyleneglycol ethers, fatty acid and polyethyleneglycol esters and block copolymers of ethylene oxide and propylene oxide, and the polyalkoxylated chain of the co-surfactant comprises from 10 to 200 ethylene oxide/propylene oxide units.

3. The immunogenic composition according to claim 1 , further comprising an immunostimulating agent.

4. The immunogenic composition according to claim 1 , further comprising:
a biological targeting ligand either grafted or not on the co-surfactant, and/or
an agent of interest selected from an optical agent or a physical agent, and/or
a cationic surfactant.

5. The immunogenic composition according to claim 1 , for which the viscosity is more than 0.1 Pa·s.

6. A drug comprising immunogenic composition of claim 1 .

7. The immunogenic composition according to claim 1 , wherein A 2 represents NH.

Assignees

• Commissariat Energie Atomique

Inventors

• Navarro Y Garcia Fabrice
• Courant Thomas
• Texier-Nogues Isabelle
• Marche Patrice

Classifications

• A61P37/04

  Immunostimulants · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61K2039/55583

  Polysaccharides · CPC title

• A61K47/545 Primary

  Heterocyclic compounds (A61K47/558 takes precedence) · CPC title