Amniotic membrane hydrogel and methods of making

Patent metadata

Field	Value
Publication number	US-10016464-B2
Application number	US-201414449867-A
Country	US
Kind code	B2
Filing date	Aug 1, 2014
Priority date	Sep 10, 2012
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

The present invention provides compositions and methods for wound healing and tissue regeneration. The compositions of the present invention comprise amniotic membrane of the placenta. In certain embodiments, the composition comprises amniotic membrane powder or solubilized amniotic membrane (SAM). In some aspects, the composition is cell-free and rich in cytokines, extracellular matrix proteins, and other components that improve tissue regeneration. In one aspect, the composition is a hydrogel scaffold that comprises amniotic membrane. The present invention reduces contraction and improves blood vessel development in regenerating tissue.

First claim

What is claimed:

1. A composition comprising amniotic membrane and a scaffold comprising a hydrogel comprising a biopolymer and a synthetic polymer;
wherein the biopolymer comprises at least one biopolymer selected from the group consisting of hyaluronan and gelatin;
wherein the synthetic polymer comprises at least one synthetic polymer selected from the group consisting of (meth)acrylate-oligolactide-PEO-oligolactide-(meth)acrylate, poly(ethylene glycol) (PEO), poly(propylene glycol) (PPO), PEO-PPO-PEO copolymers, poly(phosphazene), poly(methacrylates), poly(N-vinylpyrrolidone), PLA-PEO-PLA copolymers, PLA-PEO-PLGA copolymers, PLGA-PEO-PLA copolymers, PLGA-PEO-PLGA copolymers, poly(ethylene imine), and poly(ethylene glycol) diacrylate, wherein PLA is polylactic acid and PLGA is poly(lactide-co-glycolide); and
wherein the amniotic membrane is mixed within and dispersed in the scaffold.

2. The composition of claim 1 , wherein the hyaluronan and the gelatin are thiolated.

3. The composition of claim 1 , wherein the synthetic polymer is poly(ethylene glycol) diacrylate.

4. The composition of claim 1 , wherein the scaffold comprises a photoinitiator.

5. The composition of claim 4 , wherein the photoinitiator is 2-hydroxy-4′-hydroxyethoxy-2-methylpropiophenone.

6. The composition of claim 1 , wherein the amniotic membrane is human amniotic membrane.

7. The composition of claim 1 , wherein the composition is in the form of a solution, an ointment, a patch, a sponge, a mesh, an aerosol spray, or a foam.

8. A method of making a composition comprising amniotic membrane and a scaffold comprising a hydrogel, the method comprising:
(a) washing an amniotic membrane obtained from a mammal;
(b) lyophilizing the washed amniotic membrane;
(c) grinding the lyophilized amniotic membrane to form a powder;
(d) forming a mixture of the amniotic membrane powder, pepsin, and a solution;
(e) centrifuging the mixture to form a supernatant comprising solubilized amniotic membrane (SAM); and
(f) adding the SAM to a hydrogel to form said composition comprising said amniotic membrane and hydrogel-comprising scaffold, wherein the amniotic membrane is mixed within and dispersed in the scaffold and wherein the hydrogel comprises:
(i) at least one biopolymer selected from the group consisting of hyaluronan, chitosan, alginate, collagen, dextran, pectin, carrageenan, polylysine, gelatin, and agarose; and
(ii) at least one synthetic polymer selected from the group consisting of (meth)acrylate-oligolactide-PEO-oligolactide-(meth)acrylate, poly(ethylene glycol) (PEO), poly(propylene glycol) (PPO), PEO-PPO-PEO copolymers, poly(phosphazene), poly(methacrylates), poly(N-vinylpyrrolidone), PLA-PEO-PLA copolymers, PLA-PEO-PLGA copolymers, PLGA-PEO-PLA copolymers, PLGA-PEO-PLGA copolymers, poly(ethylene imine), and poly(ethylene glycol) diacrylate, wherein PLA is polylactic acid and PLGA is poly(lactide-co-glycolide).

9. The method of claim 8 , wherein the mammal is a human.

10. The method of claim 8 , further comprising decellularizing the amniotic membrane prior to lyophilizing.

11. The method of claim 8 , wherein the hydrogel comprises at least one biopolymer selected from the group consisting of hyaluronan and gelatin.

12. The method of claim 11 , wherein the biopolymer comprises at least hyaluronan and gelatin.

13. The method of claim 12 , wherein the hyaluronan and the gelatin are thiolated.

14. The method of claim 8 , wherein the synthetic polymer is a pegylated synthetic polymer.

15. The method of claim 8 , wherein the scaffold comprises a photoinitiator.

16. The method of claim 15 , wherein the photoinitiator is 2-hydroxy-4′-hydroxyethoxy-2-methylpropiophenone.

Assignees

• Univ Wake Forest Health Sciences

Inventors

• Murphy Sean V
• Skardal Aleksander
• Atala Anthony

Classifications

• A61K47/36

  Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• C12N2533/92

  Amnion; Decellularised dermis or mucosa · CPC title

• A61L2430/40

  Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking · CPC title

• A61L27/34

  Macromolecular materials · CPC title