Microtome chuck with light source

Patent metadata

Field	Value
Publication number	US-10012567-B2
Application number	US-201715811476-A
Country	US
Kind code	B2
Filing date	Nov 13, 2017
Priority date	Nov 14, 2016
Publication date	Jul 3, 2018
Grant date	Jul 3, 2018

Abstract

A microtome chuck including a mounting portion having a mating surface operable to removably attach the mounting portion to a sample sectioning device, and an electrical contact operable to electrically connect the mounting portion to a power source. The chuck further including a sample receiving portion coupled to the mounting portion, the sample receiving portion having a sample receiving surface dimensioned to receive a sample, a light source coupled to the sample receiving portion, the light source operable to emit a light from the sample receiving surface and through a sample positioned along the sample receiving surface, and circuitry electrically connecting the light source to the electrical contact of the mounting portion.

First claim

What is claimed is:

1. A microtome chuck comprising:
a mounting portion having a mating surface operable to removably attach the mounting portion to a sample sectioning device, and an electrical contact operable to electrically connect the mounting portion to a power source;
a sample receiving portion coupled to the mounting portion, the sample receiving portion having a sample receiving surface dimensioned to receive a sample;
a light source coupled to the sample receiving portion, the light source operable to emit a light from the sample receiving surface and through a sample positioned along the sample receiving surface; and
circuitry electrically connecting the light source to the electrical contact of the mounting portion.

2. The microtome chuck of claim 1 wherein the light source comprises a light emitting diode (LED).

3. The microtome chuck of claim 1 wherein the light source is a light emitting diode (LED) chip comprising a plurality of LEDs, and the LED chip is mounted to the sample receiving portion.

4. The microtome chuck of claim 1 wherein the light source is positioned within a cavity of the sample receiving portion, and the cavity comprises a depth substantially the same as a thickness of the light source.

5. The microtome chuck of claim 1 wherein the light source is mounted to the sample receiving surface such that the light source is positioned between a sample positioned along the sample receiving surface and the sample receiving surface.

6. The microtome chuck of claim 1 further comprising a controller operable to modify at least one of a brightness or a wavelength of the light emitted by the light source.

7. The microtome chuck of claim 6 wherein the controller modifies one of the brightness or the wavelength of the light depending upon a characteristic of the sample.

8. The microtome chuck of claim 1 wherein the circuitry comprises a flexible circuit mounted between the mounting portion and the sample receiving portion.

9. The microtome chuck of claim 1 wherein the electrical contact is a first electrical contact and the sample sectioning device comprises a second electrical contact electrically connected to the power source, and wherein when the mounting portion is attached to the sample sectioning device, the first electrical contact and the second electrical contact are in contact with one another and the light source is electrically connected to the power source.

10. The microtome chuck of claim 1 wherein the sample sectioning device comprises a manual microtome, and the power source comprises an electric current manually generated by rotating a hand wheel of the sample sectioning device.

11. A microtome comprising:
a cutting mechanism that is operable to cut sections from a sample;
a sample holder operable to hold a sample and move relative to the cutting mechanism, the sample holder having a first side and a second side, the first side faces the cutting mechanism and is dimensioned to receive a sample; and
a light source coupled to the sample holder, wherein the light source comprises an light emitting diode (LED) chip operable to emit a light from the first side of the sample holder and through a sample positioned on the first side.

12. The microtome of claim 11 wherein the LED chip is operable to emit the light at a first brightness and a second brightness different from the first brightness.

13. The microtome of claim 11 wherein the LED is a first LED, and the light source further comprises a second LED, wherein the first LED and the second LED are operable at different wavelengths.

14. The microtome of claim 11 wherein the LED is positioned on the first side of the sample holder.

15. The microtome of claim 11 further comprising an alarm operable to alert a user that the microtome is performing a cutting operation.

16. A method of controlling a microtome chuck light source comprising:
determining a characteristic of a sample held by a microtome chuck; and
controlling a characteristic of a light output from a light source of the microtome chuck based on the characteristic of the sample.

17. The method of claim 16 wherein the sample comprises a biological sample, and the characteristic of the sample comprises a color of the biological sample, a density of the biological sample, or a biological component of the biological sample.

18. The method of claim 16 wherein the light source comprises a light emitting diode (LED), and controlling the characteristic of the light output by the light source comprises changing an intensity of the light output by the LED.

19. The method of claim 16 wherein the sample comprises a biological sample embedded within paraffin, and the characteristic of the sample comprises a color of the paraffin or a density of the paraffin.

20. The method of claim 16 wherein the sample comprises a paraffin embedded biological sample and a cassette, and the characteristic of the sample comprises a color of the cassette.

21. The method of claim 16 wherein the light source comprises a plurality of light emitting diodes (LEDs) having different wavelengths, and controlling the characteristic of the light output by the light source comprises modifying an intensity of one of the light emitting diodes with respect to another of the light emitting diodes so that a desired light output color is achieved.

Assignees

• Sakura Finetek Usa Inc

Inventors

• Bui Xuan S
• Rios Alyicia Marie
• Von Bueren Erico

Classifications

• G01N2201/0627

  Use of several LED's for spectral resolution · CPC title

• G01N21/255

  Details, e.g. use of specially adapted sources, lighting or optical systems · CPC title

• G01N2001/061

  Blade details · CPC title

• B26D7/27

  Means for performing other operations combined with cutting (B26D9/00 takes precedence) · CPC title

• G01N2001/315

  Basket-type carriers for tissues · CPC title