Method to sporulate coccidial oocysts purified from animal faeces, sporulated oocysts obtained with this method and a vaccine containing these sporulated oocysts

Patent metadata

Field	Value
Publication number	US-10011815-B2
Application number	US-201515300522-A
Country	US
Kind code	B2
Filing date	Apr 2, 2015
Priority date	Apr 3, 2014
Publication date	Jul 3, 2018
Grant date	Jul 3, 2018

Abstract

The invention pertains to a method to sporulate coccidial oocysts purified from animal faeces ( 5 ), the method comprising providing the purified oocysts as a layer ( 40 ) on a supporting surface ( 22 ), maintaining the layer at least intermittently in an oxygen containing gaseous environment ( 60 ) having a relative humidity of at least 15% and maintaining the temperature of the oocysts between 19° C. and 37° C. The invention also pertains to sporulated oocysts obtained with this method and to a vaccine containing such sporulated oocysts.

First claim

The invention claimed is:

1. A method to sporulate coccidial oocysts purified from animal faeces, the method comprising providing the purified oocysts as a layer on a supporting surface, maintaining the layer at least intermittently in an oxygen containing gaseous environment having a relative humidity of at least 15% and maintaining the temperature of the oocysts between 19° C. and 37° C.

2. The method of claim 1 , wherein the thickness of the layer is below 10 mm.

3. The method of claim 1 , wherein the supporting surface is a perforated deck that is able to let water pass, but does not let the oocysts pass.

4. The method of claim 3 , wherein the supporting surface has mesh openings around a minimum dimension of the oocysts.

5. The method of claim 3 , wherein the supporting surface has mesh openings around 10 μm.

6. The method of claim 1 , wherein the supporting surface is in the form of a perforated deck that is drum shaped, and wherein the layer of oocysts is provided on the internal surface of the drum-shaped deck.

7. The method of claim 6 , wherein the drum-shaped deck is placed partly in a volume of water and partly in the gaseous environment, mounted with its longitudinal axis extending in parallel with a surface of this volume of water, wherein the deck is rotated for maintaining the layer at least intermittently in the oxygen containing gaseous environment, and wherein the drum-shaped deck is rotated around its longitudinal axis.

8. The method of claim 7 , wherein the drum-shaped deck is rotated at a circumferential speed of 5-20 rpms.

9. The method of claim 7 , wherein the volume of water is kept at a temperature between 19° C. and 37° C.

10. The method of claim 7 , wherein the volume of water is kept at a temperature of about 28° C.

11. The method of claim 1 , wherein after sporulation of the oocysts, a further aqueous medium containing an anti-infective is provided to the layer to remove contamination by a sieving step, whereafter the layer is washed to remove the anti-infective.

12. The method of claim 1 , wherein the supporting surface is a sieve deck having an effective mesh opening around the minimum dimension of the oocysts, wherein a thin layer is provided by collecting faeces of animals containing the coccidial oocysts, diluting the faeces in an aqueous medium wherein separating a coarse fraction comprising macroscopic particulate matter from the diluted faeces is optional, and collecting an aqueous fraction containing the oocysts, sieving the aqueous fraction over a first sieve deck other than the said supporting surface, the first sieve deck having mesh openings to let the oocysts pass in order to obtain an aqueous filtrate comprising the oocysts and a residue on the first sieve deck comprising particles larger than the oocysts, and then sieving the aqueous filtrate over the said supporting surface.

Assignees

• Intervet Inc

Inventors

• Schetters Theodorus Petrus Maria
• Gevers Koen

Classifications

• A61P33/02

  Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis · CPC title

• C12N1/10

  Protozoa; Culture media therefor · CPC title

• A61K39/012 Primary

  Coccidia antigens · CPC title

• A61K35/68

  Protozoa, e.g. flagella, amoebas, sporozoans, plasmodium or toxoplasma · CPC title

• C12M33/14

  with filters, sieves or membranes · CPC title