Immunological reagents and uses therefor

Patent metadata

Field	Value
Publication number	US-10011602-B2
Application number	US-201314413164-A
Country	US
Kind code	B2
Filing date	Jul 5, 2013
Priority date	Jul 6, 2012
Publication date	Jul 3, 2018
Grant date	Jul 3, 2018

Abstract

The present invention provides a ligand which binds to MR1 wherein said binding results in binding of the MR1 to MAIT cells.

First claim

The invention claimed is:

1. A method of detecting the presence of MAIT cells in a biological sample from a subject, the method comprising the steps of
a) contacting the biological sample with antigen presenting cells expressing MR1 bound to a ligand or a soluble form of MR1 bound to a ligand, under conditions that would allow binding of the MR1 or the soluble form of MR1 with MATT cells present in the sample, wherein the ligand is a compound selected from the group consisting of rRL-6HM, rRL-6AM, RL-6M, RP-5PA, 6-formyl pterin, acetyl 6-formyl pterin, 6-methyl-7-hydroxy-8-ribityl lumazine, and 6-,7-dimethyl-8-Ribityl Lumazine; and
b) detecting the presence of MAIT cell bound MR1 or MAIT cell bound soluble form of MR1 in the biological sample,
thereby detecting the presence of MAIT cells in a biological sample from a subject.

2. The method according to claim 1 , wherein the ligand is rRL-6HM.

3. The method of claim 1 , wherein the biological sample is a biological fluid.

4. The method of claim 1 , wherein the biological sample is a cell sample.

5. The method of claim 1 , wherein the biological sample is a tissue sample.

6. The method of claim 3 , wherein the biological fluid is serum, lymph, or blood.

7. The method of claim 5 , wherein the tissue sample is a bone marrow or a tissue biopsy sample including mucosal tissue.

8. The method of claim 7 , wherein the tissue biopsy sample is from the gut, gut lamina propria, or lungs.

9. The method of claim 1 , wherein the MR1 is a human, primate, or mouse MR1 polypeptide.

10. The method of claim 8 , wherein the MR1 polypeptide comprises SEQ ID NO: 1 or SEQ ID NO: 4.

11. The method of claim 1 , wherein the subject is a human.

Assignees

• Univ Melbourne
• Univ Monash
• Univ Queensland

Inventors

• Kjer-Nielsen Lars
• Mccluskey James
• Corbett Alexandra
• Rossjohn Jamie
• Onisha Patel
• Fairlie David Paul
• Liu Ligong

Classifications

• G01N2333/70539

  MHC-molecules, e.g. HLA-molecules · CPC title

• C07D475/04 Primary

  with a nitrogen atom directly attached in position 2 · CPC title

• G01N33/56972

  White blood cells · CPC title

• C07D475/02

  with an oxygen atom directly attached in position 4 · CPC title

• C07K14/70539

  MHC-molecules, e.g. HLA-molecules · CPC title