Polypeptides having beta-1,3-galactanase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-10010094-B2
Application number	US-201414909579-A
Country	US
Kind code	B2
Filing date	Aug 15, 2014
Priority date	Aug 15, 2013
Publication date	Jul 3, 2018
Grant date	Jul 3, 2018

Abstract

The present invention relates to polypeptides having beta-1,3-galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A method or producing a coffee extract, comprising the steps:
(a) providing roast and ground coffee beans;
(b) adding water and a polypeptide having beta-1,3-galactanase activity to the coffee beans, wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(c) incubating to make an aqueous coffee extract; and
(d) separating the coffee extract from the extracted coffee beans.

2. The method of claim 1 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

3. The method of claim 1 , wherein the polypeptide is the mature polypeptide of SEQ ID NO: 2.

4. The method of claim 1 , wherein the polypeptide has one or more amino acids absent from the amino and/or carboxyl terminus of the mature polypeptide of SEQ ID NO: 2.

5. The method of claim 1 , wherein the polypeptide is encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

6. The method of claim 1 , wherein the polypeptide is a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions.

Assignees

• Novozymes As

Inventors

• Spodsberg Nikolaj
• Krogh Kristian Bertel Roemer M
• Monrad Rune Nygaard

Classifications

• A23F5/28

  Drying or concentrating coffee extract {(A23F5/246 takes precedence)} · CPC title

• C12Y302/01181

  Galactan endo-beta-1,3-galactanase (3.2.1.181) · CPC title

• C12N9/2468

  acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81) · CPC title

• A23F5/246 Primary

  Addition of, or treatment with, enzymes or microorganisms (A23F5/163 and A23F5/204 take precedence) · CPC title