Method for producing cell concentrate

Patent metadata

Field	Value
Publication number	US-10006842-B2
Application number	US-201314424574-A
Country	US
Kind code	B2
Filing date	Aug 19, 2013
Priority date	Aug 30, 2012
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

Provided is a simple method for producing a cell concentrate (other than human blood) in a short time with neither loss of cells nor an excessive burden on cells. Included is a method for producing a cell concentrate using an inside-out filtration system for processing a cell suspension, the system including: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and hollow fiber separation membranes provided between the cell suspension inlet port and the cell suspension outlet port, wherein the membranes have a total cross-sectional area of 0.5-1.5 cm 2 , the membranes have an inside membrane area of 0.2 m 2 or less, the suspension is flowed inside the membranes at a linear velocity of 500-1200 cm/min, and the quotient of the division of the initial filtrate flow rate from the filtrate outlet port by the flow rate into the cell suspension inlet port is 0.4-0.7.

First claim

The invention claimed is:

1. A method for producing a cell concentrate comprising filtration of a cell suspension using an inside-out filtration system, the system comprising:
a cell suspension inlet port;
a filtrate outlet port;
a cell suspension outlet port; and
a set of hollow fiber separation membranes provided between the cell suspension inlet port and the cell suspension outlet port,
wherein
the hollow fiber separation membranes have a total cross-sectional area of 0.5 cm 2 to 1.5 cm 2 ,
the hollow fiber separation membranes have an inside membrane area of 0.2 m 2 or less,
the cell suspension is flowed inside the hollow fiber separation membranes at a linear velocity of 500 cm/min to 1200 cm/min,
the quotient of the division of an initial filtrate flow rate from the filtrate outlet port by a flow rate into the cell suspension inlet port is 0.4 to 0.7,
a cell recovery rate is at least 81%, and
a filtration time, measured from the start to the end of filtration of 1000 ml of the cell suspension, is 330 seconds or less.

2. The method for producing a cell concentrate according to claim 1 , wherein the hollow fiber separation membranes have an inner diameter of 300 μm to 1000 μm.

3. The method for producing a cell concentrate according to claim 1 , wherein the hollow fiber separation membranes have an average pore size of 0.1 μm or more.

4. The method for producing a cell concentrate according to claim 1 , wherein the hollow fiber separation membranes are made of a polysulfone-, polyolefin-, or cellulose-based material.

5. The method for producing a cell concentrate according to claim 1 ,
wherein the hollow fiber separation membranes are made of polyethersulfone.

6. The method for producing a cell concentrate according to claim 1 ,
wherein the cell suspension is an immune cell suspension.

7. A method for producing a cell concentrate, comprising the steps of:
washing a cell concentrate obtained by the production method according to claim 1 by repeating dilution with a liquid free from cells and concentration; and
further concentrating the resulting concentrate.

8. A method for cryopreserving cells, comprising cryopreserving a cell concentrate obtained by the production method according to claim 1 with liquid nitrogen.

9. The method of claim 1 , wherein the cell suspension is a human cell suspension.

10. The method of claim 9 , wherein the human cell suspension is human immune cell suspension.

Assignees

• Kaneka Corp

Inventors

• Taniguchi Shuhei

Classifications

• C12M25/10

  Hollow fibers or tubes (hollow fiber modules in general B01D63/02) · CPC title

• B01D69/081

  characterised by the fibre diameter · CPC title

• B01D63/02 Primary

  Hollow fibre modules · CPC title

• G01N2001/4088

  filtration · CPC title

• B01D61/18

  Apparatus therefor · CPC title