Soybean event SYHT04R and compositions and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-10006044-B2
Application number	US-201113994166-A
Country	US
Kind code	B2
Filing date	Dec 9, 2011
Priority date	Dec 15, 2010
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

Soybean plants comprising event SYHT04R, methods of detecting and using the same, and soybean plants comprising a heterologous insert at the same site as SYHT04R.

First claim

What is claimed is:

1. A seed of a transgenic soybean plant comprising herbicide tolerance event SYHT04R, wherein said seed comprises herbicide tolerance event SYHT04R and a representative seed comprising said event has been deposited under ATCC Accession No. PTA-10757.

2. A transgenic herbicide tolerance event SYHT04R soybean plant, cells or tissues thereof comprising nucleotides 782-2793 of SEQ ID NO: 10.

3. A nucleic acid molecule comprising SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, or SEQ ID NO: 10.

4. The nucleic acid molecule of claim 3 , wherein the nucleic acid molecule is comprised in a soybean seed deposited as ATCC Accession No. PTA-10757.

5. An amplicon comprising the nucleic acid molecule of claim 3 .

6. A seed of a transgenic soybean plant of claim 2 , wherein said seed comprises herbicide tolerance event SYHT04R and further comprises one or more additional regions encoding a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, or one or more insects, fungal, bacterial and/or viral infections.

7. The transgenic soybean seed of claim 6 , wherein the one or more additional regions encode a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides selected from group consisting of: glyphosate, glufosinate, dicamba, 2,4-D, phenoxy auxin herbicides, PPO herbicides, aryloxyphenoxypropionate herbicides, imidazolinone, sulfonyl urea, ametryne, triazine herbicides and metribuzin.

8. The transgenic soybean seed of claim 6 , wherein the one or more additional regions encode a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, the polypeptide being selected from the group consisting of: a glyphosate tolerant 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS), a glyphosate N-acetyl transferase (GAT), a herbicide tolerant 4-hydroxypyruvyldioxgenase (HPPD), a phosphinothricin acetyl transferase (PAT), a cytochrome P450, a glutathione S-transferase (GST), a herbicide tolerant acetyl-COA-carboxylase (ACCase), a herbicide tolerant acetolactate synthase (ALS), a herbicide tolerant protoporphyrinogen oxidase (PPO), a bromoxynil nitrilase, a herbicide tolerant phytoene desaturase, an aryloxyalkanoate dioxygenase, a homogentisate solanesyltransferase (HST) and a dicamba degrading enzymes.

9. The transgenic soybean seed of claim 6 , wherein the plant comprises resistance to a combination of herbicides selected from the group consisting of:
a. An HPPD inhibitor and glufosinate,
b. An HPPD inhibitor and glyphosate,
c. An HPPD inhibitor and dicamba,
d. An HPPD inhibitor and 2,4-D,
e. An HPPD inhibitor and an ALS inhibitor,
f. An HPPD inhibitor, glyphosate, and glufosinate,
g. An HPPD inhibitor, glyphosate, and dicamba,
h. An HPPD inhibitor, glyphosate, and 2,4-D,
i. An HPPD inhibitor, glyphosate, and an ALS inhibitor,
j. An HPPD inhibitor, glufosinate, and 2,4-D,
k. An HPPD inhibitor, glufosinate, and dicamba,
l. An HPPD inhibitor, glufosinate, and an ALS inhibitor,
m. An HPPD inhibitor, glyphosate, glufosinate, and dicamba,
n. An HPPD inhibitor, glyphosate, glufosinate, and 2,4-D,
o. An HPPD inhibitor, glyphosate, glufosinate, 2,4-D, and an ALS inhibitor,
p. An HPPD inhibitor, glyphosate, glufosinate, dicamba, and an ALS inhibitor,
q. An HPPD inhibitor, glyphosate, glufosinate, dicamba, 2,4-D,
r. An HPPD inhibitor, glyphosate, glufosinate, dicamba, 2,4-D, and an ALS inhibitor wherein the HPPD inhibitor comprises at least one member selected from the group consisting of isoxaflutole, bicyclopyrone, mesotrione, sulcotrione, tembotrione, topramezone pyrasulfatole and wherein the ALS inhibitor comprises at least one member selected from the group consisting of prosulfuron, primisulfuron, triasulfuron, bensulfuron, nicosulfuron, rimsulfuron, primisulfuron, thifensulfuron, foramsulfuron, chlorsulfuron, halosulfuron, imazaquin, imazapic, imazapyr, imazethapyr, imazamox, iodosulfuron, metsulfuron, mesosulfuron sulfosulfuron trifloxysulfuron tribenuron methyl, thiazopyr, diclosulam, cloransulam-methyl, flucarbazone, flumetsulam, thiencarbazone, and chlorimuron-ethyl.

10. The soybean plant of claim 2 , wherein the plant further comprises one or more additional regions encoding a polypeptide providing the plant with resistance or tolerance to one or more additional herbicides, one or more insects, or one or more fungal, bacterial and/or viral infections.

Assignees

• Hipskind John Daniel
• Burgin Kristina
• Jain Rakesh
• Terpstra Karolyn
• Dawson John
• De Framond Annick Jeanne
• Breitinger Becky
• Syngenta Participations Ag

Inventors

• Hipskind John Daniel
• Burgin Kristina
• Jain Rakesh
• Terpstra Karolyn
• Dawson John
• De Framond Annick Jeanne
• Breitinger Becky

Classifications

• C12N15/8274 Primary

  for herbicide resistance · CPC title

• C12N15/8281

  for bacterial resistance · CPC title

• A01N41/10

  Sulfones; Sulfoxides · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N15/8286

  for insect resistance · CPC title