Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

Patent metadata

Field	Value
Publication number	US-10006042-B2
Application number	US-201414904758-A
Country	US
Kind code	B2
Filing date	Aug 26, 2014
Priority date	Aug 27, 2013
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 710-1153 and 9276-15726, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-709 and 1157-9275, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1. A method of increasing growth rate, biomass, seed yield, and/or photosynthetic capacity of a plant, comprising:
(a) transforming plants with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence which is at least 95% identical to the amino acid sequence as set forth in SEQ ID NOs: 738, wherein said exogenous polynucleotide is operably linked to a heterologous promoter for directing transcription of said polynucleotide in said plants; and
(b) selecting a transgenic plant obtained from step (a) transformed with said nucleic acid construct which overexpresses said polypeptide, and exhibits increased growth rate, biomass, seed yield, and/or photosynthetic capacity as compared to a wild type plant of the same species which is grown under the same growth conditions.

2. A method of producing a crop of plants comprising growing a crop plant transformed with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence which is at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 738, wherein said exogenous polynucleotide is operably linked to a heterologous promoter, wherein the crop plant is obtained from plants which have been transformed with said nucleic acid construct which overexpresses said polypeptide in said plants, and which have been selected for increased growth rate, increased biomass, increased seed yield, and/or increased photosynthetic capacity as compared to a wild type plant of the same species which is grown under the same growth conditions, and thereby producing the crop of plants.

3. The method of claim 1 , wherein said exogenous polynucleotide comprises the nucleic acid sequence selected from the group consisting of SEQ ID NOs: 420 and 29.

4. The method of claim 1 , wherein said polypeptide has the amino acid sequence of the SEQ ID NO: 738.

5. The method of claim 2 , wherein said nucleic acid sequence encodes the amino acid sequence as set forth in SEQ ID NO: 738.

6. The method of claim 2 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 420 and 29.

7. A method of growing a crop, the method comprising seeding seeds and/or planting plantlets of a plant transformed with a nucleic acid construct comprising an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide having at least 95% amino acid sequence identity to SEQ ID NO: 738, wherein said polynucleotide is operably linked to a heterologous promoter, wherein the transformed plant is obtained from plants which have been transformed with said exogenous polynucleotide and which have been selected for at least one trait selected from the group consisting of: increased biomass, increased growth rate, increased seed yield, and increased photosynthetic capacity as compared to a wild type plant of the same species thereby growing the crop and wherein said seeds and said plantlets comprise said nucleic acid construct.

8. The method of claim 1 , wherein said selecting is performed under non-stress conditions.

Assignees

• Evogene Ltd

Inventors

• Gil Lidor
• Rimon Knopf Ronit
• Matarasso Noa
• Panik David
• Poraty-Gavra Limor
• Katz Ehud
• Karchi Hagai

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title

• C07K14/415

  from plants · CPC title