Animal feed additives comprising a protease and methods of using

Patent metadata

Field	Value
Publication number	US-10006017-B2
Application number	US-201715673594-A
Country	US
Kind code	B2
Filing date	Aug 10, 2017
Priority date	Sep 5, 2012
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.

First claim

What is claimed is:

1. An animal feed additive comprising
an S53 protease that is at least 90% identical to the polypeptide of SEQ ID NO: 5; and
at least one fat-soluble vitamin, and/or
at least one water-soluble vitamin, and/or
at least one trace mineral.

2. The animal feed additive of claim 1 , which further comprises of one or more amylases; galactanases; alpha-galactosidases; beta-glucanases; phospholipases; phytases; proteases; xylanases; or any mixture thereof.

3. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is at least 95% identical to the polypeptide of SEQ ID NO: 5.

4. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is at least 97% identical to the polypeptide of SEQ ID NO: 5.

5. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is at least 98% identical to the polypeptide of SEQ ID NO: 5.

6. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is at least 99% identical to the polypeptide of SEQ ID NO: 5.

7. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is the polypeptide of SEQ ID NO: 5.

8. The animal feed additive of claim 1 , wherein the amino acid sequence of the S53 protease is a fragment of the polypeptide of SEQ ID NO: 5.

9. An animal feed composition comprising the animal feed additive of claim 1 and having a crude protein content of 50 to 800 g/kg.

10. The animal feed composition of claim 9 , which further comprises of one or more amylases, galactanases, alpha-galactosidases, beta-glucanases, phospholipases, phytases, proteases, xylanases, or any mixture thereof.

11. A method for improving the nutritional value of a protein, comprising adding to a composition comprising at least one protein or protein source, a polypeptide comprising an S53 protease that is at least 90% identical to the amino acid sequence of SEQ ID NO: 5.

12. The method of claim 11 , wherein the protein source comprises soybean or soybean meal.

13. The method of claim 11 , wherein the amino acid sequence of the S53 protease is at least 95% identical to the polypeptide of SEQ ID NO: 5.

14. The method of claim 11 , wherein the amino acid sequence of the S53 protease is at least 96% identical to the polypeptide of SEQ ID NO: 5.

15. The method of claim 11 , wherein the amino acid sequence of the S53 protease is at least 97% identical to the polypeptide of SEQ ID NO: 5.

16. The method of claim 11 , wherein the amino acid sequence of the S53 protease is at least 98% identical to the polypeptide of SEQ ID NO: 5.

17. The method of claim 11 , wherein the amino acid sequence of the S53 protease is at least 99% identical to the polypeptide of SEQ ID NO: 5.

18. The method of claim 11 , wherein the amino acid sequence of the S53 protease is the polypeptide of SEQ ID NO: 5.

19. The method of claim 11 , wherein the amino acid sequence of the S53 protease is a fragment of the polypeptide of SEQ ID NO: 5.

20. A method for improving the nutritional value of an animal feed, comprising adding an S53 protease to the feed, wherein the S53 protease is at least 90% identical to the polypeptide of SEQ ID NO: 5.

Assignees

• Novozymes As

Inventors

• Hoff Tine
• Oestergaard Peter Rahbek
• Pontoppidan Katrine Fruergaard

Classifications

• C12P21/06

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C12N9/485 Primary

  Exopeptidases (3.4.11-3.4.19) · CPC title

• A23K20/189

  Enzymes · CPC title

• A23K10/14

  Pretreatment of feeding-stuffs with enzymes · CPC title

• A23K20/22

  Compounds of alkali metals · CPC title