Optical alignment tool

Patent metadata

Field	Value
Publication number	US-10005083-B2
Application number	US-201615382684-A
Country	US
Kind code	B2
Filing date	Dec 18, 2016
Priority date	Aug 28, 2013
Publication date	Jun 26, 2018
Grant date	Jun 26, 2018

Abstract

Provided is an inspection apparatus including: (a) a translucent or transparent plate having a bottom surface, at least a portion of the bottom surface having an opaque material printed thereon in a pattern having at least one transparent or translucent portion; and (b) a chamber disposed below the bottom surface, whereby light emitted from the chamber or through the chamber can pass through the at least one transparent or translucent portion.

First claim

What is claimed is:

1. An inspection apparatus comprising:
a translucent or transparent plate having a bottom surface, at least a portion of the bottom surface having an opaque material printed thereon in a pattern having at least one transparent or translucent portion;
a channel disposed below the bottom surface, wherein the channel comprises one or more lanes, whereby light emitted from the channel or through the channel can pass through the at least one transparent or translucent portion of the pattern;
a fluid filling at least a portion of the channel, the fluid comprising at least one light emitting material, the fluid to emit fluorescence in response to excitation light, the channel having fluid entry and exit ports that are closed.

2. The apparatus of claim 1 , wherein the fluid entry and exit ports that are closed with a sealant.

3. The apparatus of claim 1 , wherein the fluid comprises first and second fluorescent dyes to emit first and second emissions in response to first and second excitation light sources, respectively.

4. The apparatus of claim 1 , wherein the light emitting material comprises one or more fluorescent or luminescent molecules.

5. The apparatus of claim 4 , wherein the fluorescent molecules are selected from the group consisting of a Rhodamine dye and an Oxazine dye.

6. The apparatus of claim 1 , wherein the pattern comprises a plurality of translucent or transparent holes in an ordered array.

7. The apparatus of claim 1 , wherein the channel comprises a plurality of parallel lanes that are connected to form a single chamber.

8. The apparatus of claim 1 , wherein the channel includes a detection lane positioned between an ingress lane and an egress lane, the ingress and egress lanes having a length to prevent bubbles from encroaching into the detection lane.

9. The apparatus of claim 8 , wherein the detection lane that is relatively wide compared to the ingress lane and the egress lane that are relatively narrow.

10. The apparatus of claim 8 , wherein the ingress and egress lanes connect the detection lanes to ingress and egress ports respectively.

11. A method of evaluating an imaging module comprising:
positioning an imaging module in optical alignment with an inspection apparatus that comprises: (a) a translucent or transparent plate having a bottom surface, at least a portion of the bottom surface having an opaque material printed thereon in a pattern having at least one transparent or translucent portion; (b) a channel disposed below the bottom surface, wherein the channel comprises one or more lanes, whereby light emitted from the channel or through the channel can pass through the at least one transparent or translucent portion of the pattern; and (c) a fluid filling at least a portion of the channel, the fluid comprising at least one light emitting material, the fluid to emit fluorescence in response to excitation light, the channel having fluid entry and exit ports;
detecting light transmitted through one or more of the transparent or translucent portions; and
based on the light that is detected, performing at least one of: i) determining an accuracy of the optical alignment based on the light that is detected, ii) determining an autofocus accuracy of a detector that detects the light that is detected, or iii) finding a fiducial on the inspection apparatus based on the light that is detected.

12. The method of claim 11 , further comprising setting excitation source currents based on the light that is detected when using the excitation source to irradiate the inspection apparatus.

13. The method of claim 11 , further comprising calibrating an excitation source based on the light that is detected when using the excitation source to irradiate the inspection apparatus.

14. The method claim 11 , further comprising calibrating a detector that detects the light that is detected.

15. The method of claim 11 , further comprising determining at least one of:
i) an image uniformity correction or flat field correction based on the light that is detected;
ii) z bias in an excitation source based on the light that is detected when using the excitation source to irradiate the inspection apparatus;
iii) camera-to-camera xy offset based on the light that is detected;
iv) repeatability of xy stage positioning or hysteresis in xy stage positioning based on the light that is detected; or
v) focus repeatability based on the light that is detected.

16. The method of claim 11 , wherein the fluid entry and exit ports that are closed with a sealant.

17. The method of claim 11 , wherein the light emitting material comprises one or more fluorescent or luminescent molecules.

18. The method of claim 11 , wherein the pattern comprises a plurality of translucent or transparent holes in an ordered array.

19. The method of claim 11 , wherein the channel comprises a plurality of parallel lanes that are connected to form a single chamber.

20. The method of claim 11 , wherein the channel includes a detection lane positioned between an ingress lane and an egress lane, the ingress and egress lanes having a length to prevent bubbles from encroaching into the detection lane.

Assignees

• Illumina Inc

Inventors

• Earney John
• Watson Dakota
• Pinto Joseph

Classifications

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N21/278

  Constitution of standards · CPC title

• G01N2021/6482

  Sample cells, cuvettes · CPC title

• G01N2021/6439

  with indicators, stains, dyes, tags, labels, marks · CPC title

• G01N2021/058

  Flat flow cell · CPC title