Apparatus and method for detecting microbes or bacteria

Patent metadata

Field	Value
Publication number	US-10001467-B2
Application number	US-201615170508-A
Country	US
Kind code	B2
Filing date	Jun 1, 2016
Priority date	Nov 17, 2015
Publication date	Jun 19, 2018
Grant date	Jun 19, 2018

Abstract

An apparatus and method for detecting microbes use laser speckles. The apparatus includes a light source configured to irradiate light into a sample to detect microbes, and a measuring part configured to measure laser speckles, which are formed due to a multiple scattering of the light which is incident into the sample, every reference time and to measure concentration of the microbes contained in the sample based on temporal correlation of the measured laser speckles.

First claim

What is claimed is:

1. An apparatus for detecting microbes, the apparatus comprising:
a light source configured to irradiate light into a sample to detect microbes; and
a measuring part configured to measure laser speckles, which are formed due to a multiple scattering of the light which is incident into the sample, every reference time and to measure concentration of the microbes, which are contained in the sample, based on temporal correlation of the measured laser speckles, wherein the measuring part is configured to measure laser speckles based on constructive interference and destructive interference that vary with activity of microbes contained in the sample, and
wherein the measuring part is configured to calculate a temporal correlation coefficient based on temporal correlation of the measured laser speckles and measure concentration of the microbes, which are contained in the sample, using an analysis for a time when the temporal correlation coefficient is equal to or lower than a specific value.

2. The apparatus of claim 1 , wherein the measuring part is configured to measure the concentration of the microbes by analyzing a variation of a pattern of the laser speckles that are measured every reference time.

3. The apparatus of claim 1 , wherein the measuring part is configured to measure a distribution of the microbes, which are contained in the sample, based on a standard deviation of light intensity of the laser speckles.

4. The apparatus of claim 1 , wherein the measuring part is configured to calculate a standard deviation of light intensity based on the constructive interference and the destructive interference.

5. The apparatus of claim 1 , wherein the light source and the measuring part are restrictive in motion until the light is irradiated into the sample and the concentration of the microbes is measured.

6. The apparatus of claim 1 , wherein the light source and the measuring part are configured to construct a reflective optical system.

7. The apparatus of claim 1 , wherein the light source and the measuring part are configured to construct a transmittal optical system.

8. The apparatus of claim 1 , wherein the light source and the measuring part are configured in a package type.

9. The apparatus of claim 1 , further comprising:
a beam splitter configured to change a path of light that is incident on the sample and reflectively exits from the sample through a multiple scattering.

10. A method for detecting microbes, the method comprising:
irradiating light into a sample to detect microbes;
measuring laser speckles, which are formed due to a multiple scattering of the light incident on the sample, every reference time, wherein the laser speckles are measured based on constructive interference and destructive interference that vary with activity of microbes contained in the sample; and
measuring concentration of microbes, which are contained in the sample, based on temporal correlation of the measured laser speckles,
wherein the measuring concentration of microbes comprises measuring laser speckles based on constructive interference and destructive interference that vary with activity of microbes contained in the sample, and
wherein the measuring concentration of microbes comprises calculating a temporal correlation coefficient based on temporal correlation of the measured laser speckles and measuring concentration of the microbes, which are contained in the sample, using an analysis for a time when a temporal correlation coefficient is equal to or lower than a specific value.

11. The method of claim 10 , wherein the measuring of the concentration of the microbes comprises:
measuring the concentration of the microbes by analyzing a variation of the laser speckles measured every reference time.

12. The method of claim 10 , wherein the measuring of the concentration of the microbes comprises:
measuring a distribution of bacteria, which are contained in the sample, based on a standard deviation of light intensity of the laser speckles.

13. The method of claim 10 , wherein the measuring of the concentration of the microbes comprises:
measuring a standard deviation of light intensity based on the constructive interference and the destructive interference.

Assignees

• Korea Advanced Inst Sci & Tech
• Korea Advancad Institute Of Science And Tech

Inventors

• Park Yongkeun
• Yoon Jonghee
• Lee KyeoReh

Classifications

• G01N2201/066

  Modifiable path; multiple paths in one sample · CPC title

• G01N21/49

  within a body or fluid · CPC title

• G01N33/483 Primary

  Physical analysis of biological material · CPC title

• G01N2201/06113

  Coherent sources; lasers · CPC title

• G01N33/02

  Food · CPC title