Cellular probe device, system and analysis method

Patent metadata

Field	Value
Publication number	US-10000789-B2
Application number	US-201514967071-A
Country	US
Kind code	B2
Filing date	Dec 11, 2015
Priority date	Jun 17, 2014
Publication date	Jun 19, 2018
Grant date	Jun 19, 2018

Abstract

A sampling probe, system and analysis method is disclosed. The sampling probe in one embodiment is constructed of a silicon substrate having a first channel, a second channel, a first tapered tip with an opening, and a second tapered tip with an opening, wherein the first channel extends from the first tapered tip opening to the second tapered tip opening, and wherein the second channel extends from an inlet port to a junction with the first channel.

First claim

What is claimed is:

1. A sampling probe, comprising:
a polymeric substrate having a first bore and a second bore which intersect substantially perpendicular at a junction, the first bore having a first tapered capillary tip extending from a first end thereof, and a second tapered capillary tip extending from a second end thereof, wherein the junction is at a position between the first tapered capillary tip and the second tapered capillary tip such that a solvent introduced into the second bore flows through the junction into the first bore.

2. The sampling probe of claim 1 , wherein the polymeric substrate is constructed of a pair of flat polymeric sheets bonded together.

3. The sampling probe of claim 1 in fluid communication with a mass spectrometer via the second tapered capillary tip, and in fluid communication with a solvent source via the second bore, forming a sample analysis system.

4. A sampling probe, comprising: a silicon substrate having a first channel, a second channel, a first tapered tip with an opening, and a second tapered tip with an opening, wherein the first channel extends from the first tapered tip opening to the second tapered tip opening, and wherein the second channel extends substantially perpendicular from an inlet port to a junction with the first channel.

5. The sampling probe of claim 4 , wherein the junction of the first channel and the second channel is in the first tapered tip.

6. The sampling probe of claim 4 , wherein the junction of the first channel and the second channel is at a position between the first tapered tip and the second tapered tip.

7. The sampling probe of claim 4 , wherein the silicon substrate is constructed of a pair of flat silicon sheets bonded together.

8. The sampling probe of claim 4 in fluid communication with a mass spectrometer via the second tapered tip, and in fluid communication with a solvent source via the second channel, forming a sample analysis system.

9. A method of sampling a cell or tissue source, comprising: obtaining a sample from the cell or tissue source via a sample analysis system comprising a sampling probe, wherein the sampling probe comprises:
(1) a polymeric substrate having a first bore and a second bore which intersect substantially perpendicular at a junction, the first bore having a first tapered capillary tip extending from a first end thereof, and a second tapered capillary tip extending from a second end thereof, wherein the junction is at a position between the first tapered capillary tip and the second tapered capillary tip such that a solvent introduced into the second bore flows through the junction into the first bore; and/or
(2) a silicon substrate having a first channel, a second channel, a first tapered tip with an opening, and a second tapered tip with an opening, wherein the first channel extends from the first tapered tip opening to the second tapered tip opening, and wherein the second channel extends from an inlet port to a junction with the first channel.

Assignees

• Univ Oklahoma

Inventors

• Yang Zhibo
• Liu Renmeng
• Pan Ning
• Rao Wei
• Vowcicefski Rachel M

Classifications

• H01J49/0004

  Imaging particle spectrometry · CPC title

• G01N2001/028

  Sampling from a surface, swabbing, vaporising · CPC title

• G01N1/04

  in the solid state, e.g. by cutting · CPC title

• G01N1/02

  Devices for withdrawing samples {(sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08)} · CPC title

• H01J49/0404 Primary

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title