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Transformant for expressing cis-prenyltranferase and Nogo B receptor
US10000774B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10000774-B2 |
| Application number | US-201515107178-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 6, 2015 |
| Priority date | Jan 20, 2014 |
| Publication date | Jun 19, 2018 |
| Grant date | Jun 19, 2018 |
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Abstract
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Provided are a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor, which are considered to be involved in polyisoprenoid biosynthesis, into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor, and a method for producing a polyisoprenoid using the transformant. The present invention relates to a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor.
First claim
Opening claim text (preview).
The invention claimed is: 1. A transformant of a Hevea or Taraxacum cell, produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor into a Hevea or Taraxacum cell for the cell to express the cis-prenyltransferase and the Nogo-B receptor, wherein the gene coding for a Nogo-B receptor is either of the following DNAs: a DNA comprising the nucleotide sequence of SEQ ID NO: 5; and a DNA having at least 90% sequence identity with the nucleotide sequence of SEQ ID NO: 5, wherein the gene coding for a cis-prenyltransferase is either of the following DNAs: a DNA comprising the nucleotide sequence of SEQ ID NO: 1 or 3; and a DNA having at least 90% sequence identity with the nucleotide sequence of SEQ ID NO: 1 or 3. 2. The transformant according to claim 1 , wherein at least one of the gene coding for a cis-prenyltransferase or the gene coding for a Nogo-B receptor is derived from a plant. 3. The transformant according to claim 2 , wherein at least one of the gene coding for a cis-prenyltransferase or the gene coding for a Nogo-B receptor is derived from an isoprenoid-producing plant. 4. The transformant according to claim 3 , wherein at least one of the gene coding for a cis-prenyltransferase or the gene coding for a Nogo-B receptor is derived from Hevea brasiliensis. 5. The transformant according to claim 1 , wherein the gene coding for a cis-prenyltransferase is either of the following DNAs: a DNA comprising the nucleotide sequence of SEQ ID NO: 1; and a DNA having at least 90% sequence identity with the nucleotide sequence of SEQ ID NO: 1. 6. A method for producing a polyisoprenoid using the transformant according to claim 1 .
Assignees
Inventors
Classifications
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation · CPC title
- C07K14/705Primary
Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} · CPC title
transferring alkyl or aryl groups other than methyl groups (2.5) · CPC title
- C12P5/007Primary
containing one or more isoprene units, i.e. terpenes (carotenes C12P23/00) · CPC title
Rubber cis-polyprenylcistransferase (2.5.1.20) · CPC title
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- What does patent US10000774B2 cover?
- Provided are a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor, which are considered to be involved in polyisoprenoid biosynthesis, into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor, and a method for producing a polyisoprenoid using the transformant. The present invention relates to a…
- Who is the assignee on this patent?
- Sumitomo Rubber Ind
- What technology area does this patent fall under?
- Primary CPC classification C07K14/705. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 19 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).